Acquisition of a 9.4 Tesla Magnet for Solid State NMR

为组装固体核磁共振谱仪，提出了获取大口径9.4特斯拉磁体的建议。本研究针对蛋白质复合体(胶原蛋白)、蛋白质脂复合体(脂质双层中的Gramiidin A)和DNA液晶相的结构和动力学特性进行了研究。在时间允许的情况下，个人将在广泛的学科中进一步使用这一工具。固体核磁共振开始在生物物理学中发挥重要作用，特别是在动力学领域。动态表征的其他方法不能产生与固态核磁共振相同的细节。从特定位置标记的生物分子可以确定具有原子分辨率的分子运动模型。该模型包括对发生运动的轴的描述、运动的类型、运动是扩散的还是不连续的、运动的幅度，最后是运动的频率。有了这样的动力学特征，获得动力学和函数之间的相关性的可能性非常高。在FSU设施中目前的相对低场(4.7特斯拉)固态核磁共振仪器上，这里描述的许多具体实验是不可能的，这不仅是因为低场，而且因为它可用的时间非常有限。基于改进的灵敏度(Boltzman分布)、改善的分辨率(通过最小化13C谱与场相关的14N线展宽效应)、低旋磁比核的观测、减少声振铃(对于2H谱)以及观测具有非整数自旋的四极核，给出了高场(9.4特斯拉)仪器的详细理由。

Acquisition of a wide bore 9.4 Tesla magnet for the assembly of a solid state NMR spectrometer is proposed. This research is directed toward the structural and dynamic characterization of protein complexes (collagen), protein lipid complexes (Gramicidin A in a lipid bilayer) and DNA liquid crystalline phases. Further use of the instrument will be made by individuals in a wide range of disciplines as time permits. Solid state NMR is beginning to play a major role in biophysics, especially in the realm of dynamics. The other approaches to dynamic characterizations do not yield the same sort of detail that is possible with solid state NMR. It is possible from specific site labeled biomolecules to determine a model for molecular motions with atomic resolution. This model includes a description of the axis about which the motion is occurring, the type of motion, whether it be diffusional or discontinuous, the amplitude of the motion and finally the frequency of the motion. With such characterizations of the dynamics the potential for obtaining correlations between dynamics and function is very high. Many of the specific experiments described herein would not be possible on the present relatively low field (4.7 Tesla) solid state NMR instrumentation in the FSU facility, not only because of the low field but also because of the severely limited time available on it. A detailed justification for a high field (9.4 Tesla) instrument is given, based on improved sensitivity (Boltzman distribution), improved resolution (by minimizing field dependent 14 N line broadening effects on the 13 C spectra), observation of low gyromagnetic ratio nuclei, reduced acoustic ringing (for 2 H spectra), and observation of quadrupolar nuclei with nonintegral spin.