Acquisition of a 9.4 Tesla Magnet for Solid State NMR

收购宽孔9.4特斯拉磁铁组装固态核磁共振光谱仪提出。本研究针对蛋白质复合物（胶原蛋白）、蛋白质脂质复合物（脂质双分子层中的Gramicidin A）和DNA液晶相的结构和动态表征。在时间允许的情况下，广泛学科的个人将进一步使用该工具。固体核磁共振正开始在生物物理学，特别是在动力学领域发挥重要作用。动态表征的其他方法不能产生固态核磁共振可能产生的相同类型的细节。这是可能的，从特定的位置标记的生物分子，以确定分子运动的原子分辨率模型。这个模型包括对运动发生的轴的描述，运动的类型，是扩散的还是不连续的，运动的幅度，最后是运动的频率。有了这样的动力学特征，获得动力学和函数之间的相关性的可能性是非常高的。本文所描述的许多具体实验在FSU设施中目前相对较低的场（4.7特斯拉）固态核磁共振仪器上是不可能的，这不仅是因为低场，还因为它的可用时间严重有限。基于提高灵敏度（玻尔兹曼分布）、提高分辨率（通过最小化场相关的14n线加宽对13c光谱的影响）、观测低回旋磁比核、减少声波振铃（对于2h光谱）、以及非积分自旋的四极核的观察。

Acquisition of a wide bore 9.4 Tesla magnet for the assembly of a solid state NMR spectrometer is proposed. This research is directed toward the structural and dynamic characterization of protein complexes (collagen), protein lipid complexes (Gramicidin A in a lipid bilayer) and DNA liquid crystalline phases. Further use of the instrument will be made by individuals in a wide range of disciplines as time permits. Solid state NMR is beginning to play a major role in biophysics, especially in the realm of dynamics. The other approaches to dynamic characterizations do not yield the same sort of detail that is possible with solid state NMR. It is possible from specific site labeled biomolecules to determine a model for molecular motions with atomic resolution. This model includes a description of the axis about which the motion is occurring, the type of motion, whether it be diffusional or discontinuous, the amplitude of the motion and finally the frequency of the motion. With such characterizations of the dynamics the potential for obtaining correlations between dynamics and function is very high. Many of the specific experiments described herein would not be possible on the present relatively low field (4.7 Tesla) solid state NMR instrumentation in the FSU facility, not only because of the low field but also because of the severely limited time available on it. A detailed justification for a high field (9.4 Tesla) instrument is given, based on improved sensitivity (Boltzman distribution), improved resolution (by minimizing field dependent 14 N line broadening effects on the 13 C spectra), observation of low gyromagnetic ratio nuclei, reduced acoustic ringing (for 2 H spectra), and observation of quadrupolar nuclei with nonintegral spin.