Plant Culture Systems for in vitro Production of Secondary Metabolities

用于体外产生次生代谢的植物培养系统

• 批准号: 9009526
• 负责人: Susan Harlander
• 金额: $ 6.85万
• 依托单位: University of Minnesota Saint Paul
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9009526&HistoricalAwards=false
• 关键词: Plant; Culture; Systems; vitro; Production

Plants are used as the source of many useful compounds and mixtures including pharmaceuticals, flavors and fragrances, essential oils, enzymes, and pigments. Large markets exist for these secondary plant metabolites which are normally obtained by extraction from intact plants. Plant tissue culture (PTC) may provide an alternative to whole plants as biological sources of these useful compounds. PTC is a technique which allows plant cells to be grown in solid or liquid medium in the laboratory under carefully controlled environmental conditions. The purpose of this study is to develop a model system for studying the potential of strawberry cells grown in culture to produce flavors and pigments of interest to the food processing industry as natural food ingredients. The investigations will include the production of the anticarcinogenic compound, ellagic acid, by strawberry cell suspension cultures. Factors that influence the kinetics of growth and secondary metabolite production of strawberry cells will be systematically studied using suspension cultures propagated in roller bottle and low- shear cell culture bioreactors. Information obtained in this study will be applicable to other plant cell culture systems.

植物被用作许多有用的化合物和混合物的来源，包括药物、香精和香料、精油、酶和色素。这些次生植物代谢物存在很大的市场，通常通过从完整的植物中提取而获得。植物组织培养（PTC）可能提供替代整个植物作为这些有用化合物的生物来源。PTC是一种允许植物细胞在实验室的固体或液体培养基中在严格控制的环境条件下生长的技术。本研究的目的是开发一个模型系统，用于研究草莓细胞在培养中产生食品加工业感兴趣的香料和色素作为天然食品成分的潜力。调查将包括生产的抗癌化合物，鞣花酸，草莓细胞悬浮培养。本文将采用滚轮瓶和低剪切细胞培养生物反应器进行悬浮培养，系统研究影响草莓细胞生长动力学和次生代谢物产生的因素。本研究获得的信息将适用于其他植物细胞培养系统。