Clathrate Hydrates in Protein-Containing Reversed Micellar System

本研究探讨了反胶束溶液中笼形物水合物的形成对蛋白质溶解度和构象的影响。初步结果表明，在常规相转移方法无效的情况下，水合物的形成可以在一定条件下影响蛋白质的胶束溶解。还发现水合物形成是一种原位修饰胶束大小的方法，从而改变了蛋白质的构象和酶的活性。因此，提出了一套相关的研究，利用这些概念来提高蛋白质的提取成反胶束，并以适当的形式回收。还提出了利用水合物来改变反胶束中酶的催化活性。到目前为止，对反胶束中水合物形成现象的研究基本上是空白的。本研究在很大程度上是现象学的，某些方面是探索性的。

This research investigates the effects of clathrate hydrate formation in reversed micellar solutions on the solubility and conformation of proteins. Preliminary results show that hydrate formation can, under certain conditions, effect protein solubilization into micelles when conventional phase transfer methods prove ineffective. It has also been found that hydrate formation is a method of in-situ modification of micelle size, and thereby, of protein conformation and enzyme activity. Therefore a set of related studies is proposed that exploit these concepts to enhance protein extraction into reversed micelles, and recovery in appropriate form. The use of hydrates to alter the catalytic activity of enzymes in reversed micelles is also proposed. The phenomenon of hydrate formation in reversed micelles is essentially unstudied up to the present. To a large extent, this research is phenomenological, and certain aspects are exploratory.