Proteases from Hyperthermophilic Archaebacteria: Physiolo- gical, Genetic, Biochemical and Engineering Studies
来自超嗜热古细菌的蛋白酶:生理学、遗传学、生物化学和工程研究
基本信息
- 批准号:9011583
- 负责人:
- 金额:$ 41.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1990
- 资助国家:美国
- 起止时间:1990-09-01 至 1992-09-01
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Bacteria able to grow at temperatures at or above 100 degrees C, i.e., hyperthermophilic archaebacteria, have only recently been isolated from a variety of geothermal locations. The work proposed here addresses physiological, genetic, biochemical and engineering issues for proteolytic enzymes produced by Pyrococcus furiosus, which has an optimal growth at 100 degrees C. Initial studies will focus on the use of hyperthermophilic proteases as alternatives to those currently used in biochemical processing. In addition, issues related to the extreme thermostability of thermal stable proteases will be investigated to attempt to determine the structural basis for function at elevated temperatures.
能够在100摄氏度或以上的温度下生长的细菌,即超嗜热古细菌,直到最近才从各种地热地点分离出来。本文提出的工作解决了由焦球菌(Pyrococcus furiosus)产生的蛋白水解酶的生理、遗传、生化和工程问题,这种酶在100℃下生长最佳。初步研究将集中在使用超嗜热蛋白酶作为目前生化处理中使用的蛋白酶的替代品。此外,与热稳定蛋白酶的极端热稳定性有关的问题将被研究,以试图确定在高温下功能的结构基础。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Robert Kelly其他文献
The Impact of Sociodemographic Variables on Functional Recovery Following Lower Extremity Amputation
- DOI:
10.1016/j.jvs.2024.06.094 - 发表时间:
2024-09-01 - 期刊:
- 影响因子:
- 作者:
Rylie O'Meara;Karan Chawla;Akshita Gorantla;Robert Kelly;Matthew DeJong;Trissa Babrowski;Pegge Halandras;Matthew Blecha - 通讯作者:
Matthew Blecha
0177 : Deciphering multiple roles of Nkx2-5 during ventricular non-compaction
- DOI:
10.1016/s1878-6480(16)30468-2 - 发表时间:
2016-04-01 - 期刊:
- 影响因子:
- 作者:
Caroline Choquet;Minh Nguyen;Frank Kober;Monique Bernard;Robert Kelly;Nathalie Lalevee;Lucile Miquerol - 通讯作者:
Lucile Miquerol
Effect of pH and Al Cations on Chromate Inhibition of Galvanic-Induced Corrosion of AA7050-T7451 Macro-Coupled to 316SS
pH 和 Al 阳离子对 AA7050-T7451 与 316SS 宏观耦合的电偶腐蚀铬酸盐抑制的影响
- DOI:
10.1149/1945-7111/ac412a - 发表时间:
2021 - 期刊:
- 影响因子:3.9
- 作者:
Utibe;C. Glover;J. Scully;Robert Kelly - 通讯作者:
Robert Kelly
Kiosk 11R-TA-07 - T1휌 Imaging for Detecting Takotsubo Cardiomyopathy
11R-TA-07 自助服务终端 - T1ρ成像用于检测应激性心肌病
- DOI:
10.1016/j.jocmr.2024.100300 - 发表时间:
2024-03-01 - 期刊:
- 影响因子:6.100
- 作者:
Liene Balode;James Ross;Dana Dawson;David Gamble;Robert Kelly - 通讯作者:
Robert Kelly
0269: Vascular remodeling of the endocardium following cardiac infarction occured by arteriogenesis and angiogenesis
- DOI:
10.1016/s1878-6480(14)71322-9 - 发表时间:
2014-04-01 - 期刊:
- 影响因子:
- 作者:
Lucile Miquerol;Cécile Cassan;Jerome Thireau;Sylvain Richard;Robert Kelly - 通讯作者:
Robert Kelly
Robert Kelly的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Robert Kelly', 18)}}的其他基金
Population Growth and Human Behavioral Change
人口增长和人类行为变化
- 批准号:
1939019 - 财政年份:2020
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
Populating a Radiocarbon Database of North America, Phase III
填充北美放射性碳数据库,第三阶段
- 批准号:
1822033 - 财政年份:2018
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
Leveraging Extreme Thermoacidophily for Bio-based Chemicals
利用生物基化学品的极端嗜热性
- 批准号:
1802939 - 财政年份:2018
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
Populating a Radiocarbon Database of North American, Phase II
填充北美放射性碳数据库,第二阶段
- 批准号:
1624061 - 财政年份:2016
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
Populating a Radiocarbon Database of Western North America
填充北美西部的放射性碳数据库
- 批准号:
1418858 - 财政年份:2014
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
COLLABORATIVE RESEARCH: Exploiting microbial hyperthermophilicity to produce an industrial chemical
合作研究:利用微生物的超嗜热性来生产工业化学品
- 批准号:
1264052 - 财政年份:2013
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
Doctoral Dissertation Improvement Grant: Testing Colonization Models in New England
博士论文改进补助金:在新英格兰测试殖民模型
- 批准号:
1342656 - 财政年份:2013
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
Human Occupation of the Bighorn Mountains
人类对大角山的占领
- 批准号:
0710868 - 财政年份:2007
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
Functional Approaches for Annotating Secretome-bound Small ORFs in Microbial Genomes
注释微生物基因组中分泌蛋白组结合的小 ORF 的功能方法
- 批准号:
0730091 - 财政年份:2007
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
Collaborative Research: Biotransformations Near and Above 100°C: Hyperthermophilic Microorganisms and Enzymes for Bioenergy Conversion
合作研究:接近和高于 100°C 的生物转化:用于生物能源转化的超嗜热微生物和酶
- 批准号:
0617272 - 财政年份:2006
- 资助金额:
$ 41.29万 - 项目类别:
Standard Grant
相似海外基金
Functional and structural analysis of proline dehydrogenase for elucidation of proline biosynthesis in hyperthermophilic archaea
脯氨酸脱氢酶的功能和结构分析,用于阐明超嗜热古菌中脯氨酸的生物合成
- 批准号:
23K05658 - 财政年份:2023
- 资助金额:
$ 41.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Revival of a hyperthermophilic-like biotic carbon capture through rational-design protein engineering
通过合理设计的蛋白质工程复兴类超嗜热生物碳捕获
- 批准号:
568880-2022 - 财政年份:2022
- 资助金额:
$ 41.29万 - 项目类别:
Postgraduate Scholarships - Doctoral
Life cycle of hyperthermophilic filamentous phage reveal to horizontal gene transfer and evolution in extreme environment
超嗜热丝状噬菌体的生命周期揭示了极端环境中的水平基因转移和进化
- 批准号:
20H02903 - 财政年份:2020
- 资助金额:
$ 41.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of binding scaffold with a protein from a hyperthermophilic archaeon
用来自超嗜热古菌的蛋白质开发结合支架
- 批准号:
19K05827 - 财政年份:2019
- 资助金额:
$ 41.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Elucidation of the life-sustaining mechanism of hyperthermophilic archaea without DNA replication origin
阐明无DNA复制起点的超嗜热古菌的生命维持机制
- 批准号:
19K22289 - 财政年份:2019
- 资助金额:
$ 41.29万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Exploration of carbohydrate metabolism in hyperthermophilic archaea: novel approaches, enzymes and metabolic pathways
超嗜热古菌碳水化合物代谢的探索:新方法、酶和代谢途径
- 批准号:
381206548 - 财政年份:2018
- 资助金额:
$ 41.29万 - 项目类别:
Research Grants
Development of an artificial metalloenzyme based on a peroxiredoxin from hyperthermophilic archaea
基于超嗜热古菌过氧化还原蛋白的人工金属酶的开发
- 批准号:
19K21133 - 财政年份:2018
- 资助金额:
$ 41.29万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
Replication mechanism of hyperthermophilic Clavaviridae virus APBV1
超嗜热棒状病毒APBV1的复制机制
- 批准号:
18K14372 - 财政年份:2018
- 资助金额:
$ 41.29万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Cellular membrane engineering of Escherichia coli using membrane lipids from hyperthermophilic archaea
使用来自超嗜热古菌的膜脂对大肠杆菌进行细胞膜工程
- 批准号:
18K19170 - 财政年份:2018
- 资助金额:
$ 41.29万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Elucidation of DNA repair pathway involving the novel endonuclease derived from hyperthermophilic archaea
阐明涉及源自超嗜热古菌的新型核酸内切酶的 DNA 修复途径
- 批准号:
18K05442 - 财政年份:2018
- 资助金额:
$ 41.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)