Proteases from Hyperthermophilic Archaebacteria: Physiolo- gical, Genetic, Biochemical and Engineering Studies

能在100度或以上的温度下生长的细菌 摄氏度，即，超嗜热古细菌，只有 最近从各种地热中分离出来 地点 这里提出的工作涉及生理， 蛋白水解的遗传、生物化学和工程问题 由激烈火球菌产生的酶，其具有 最佳生长温度为100摄氏度。 初步研究将侧重于 使用超嗜热蛋白酶作为替代物 目前用于生化处理的那些。 在 此外，与热稳定性有关的问题 将研究热稳定蛋白酶， 确定功能的结构基础， 温度

Bacteria able to grow at temperatures at or above 100 degrees C, i.e., hyperthermophilic archaebacteria, have only recently been isolated from a variety of geothermal locations. The work proposed here addresses physiological, genetic, biochemical and engineering issues for proteolytic enzymes produced by Pyrococcus furiosus, which has an optimal growth at 100 degrees C. Initial studies will focus on the use of hyperthermophilic proteases as alternatives to those currently used in biochemical processing. In addition, issues related to the extreme thermostability of thermal stable proteases will be investigated to attempt to determine the structural basis for function at elevated temperatures.