Leveraging Extreme Thermoacidophily for Bio-based Chemicals

利用生物基化学品的极端嗜热性

基本信息

  • 批准号:
    1802939
  • 负责人:
  • 金额:
    $ 30.15万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-07-01 至 2023-06-30
  • 项目状态:
    已结题

项目摘要

Sulfur compounds and carbon dioxide are generated in copious amounts as byproducts of natural gas and petroleum refining processes, which creates significant challenges in their safe handling and economical disposal. Fortunately, microorganisms have much to offer toward the development of environmentally-sound and energy-efficient processes to recycle these industrial waste streams. Sulfur and carbon dioxide also happen to be potential sources of energy and carbon for microorganisms called thermoacidophilic archaea that grow in extremely hot, acidic environments. One potential approach to dealing with the excess industrial sulfur and carbon dioxide is to engineer the metabolic pathways of these archaea such that they use the byproducts to produce chemicals of commercial value. This project will apply recently developed tools for genetically engineering extreme thermoacidophiles to create novel microorganisms. The study will examine the hypothesis that inserting sulfur oxidation genes from one organism into another will result in a new microorganism capable of converting carbon dioxide and sulfur into industrially-relevant organic chemicals. Engineering sulfur oxidation genes into microorganisms that naturally thrive under extreme conditions has many potential benefits. The ability to grow at hot and acidic conditions minimize risk of contamination by undesirable microorganisms and enables the bioprocess to be operated much like a conventional chemical process. The high heat tolerance of the microorganisms is also advantageous in recovering biochemical products with low boiling points. To broaden the educational impact and inclusiveness of this study, faculty and students at the North Carolina School for the Deaf (NCSD) will participate through biotechnology-based teaching modules. The modules will incorporate closed-captioning and other features to facilitate educational objectives for the deaf and hearing-impaired. Modules will be demonstrated on-site at NCSD. Additionally, the NCSU Biotechnology Program will host visiting middle school and high school students from NCSD for a day-long laboratory experience that will also familiarize them with higher educational opportunities. The objective of this project is to metabolically engineer strains of a model thermoacidophilic archaeon, Sulfolobus acidocaldarius DSM639, to produce chemicals from carbon dioxide (CO2) and reduced, inorganic sulfur compounds (RISCs). Pangenomic analysis of the extremely thermoacidophilic archaea revealed the presence of a novel CO2 fixation cycle and key genes that can be recruited to enable S. acidocaldarius to oxidize sulfur. These unique biological characteristics will be exploited in the metabolic engineering of the naturally heat- and acid-tolerant organism. Metabolic engineering approaches will be informed by fundamental assessments of wild-type and recombinant S. acidocaldarius physiology. The specific objectives of this project are: (1) Create and characterize engineered S. acidocaldarius DSM639 strains that utilize CO2 as a carbon source and RISCs as energy sources. S. acidocaldarius encodes a cycle for CO2 fixation and an incomplete pathway for sulfur oxidation that can be repaired with genes recruited from chemolithoautotrophic Sulfolobales. (2) Demonstrate that a functional, biosynthetic, thermophilic pathway for acetone can be engineered into strains of S. acidocaldarius DSM639. Genes/enzymes have been identified that can be recruited from other thermophilic microorganisms to enable S. acidocaldarius to produce an industrial chemical from simple sugars. (3) Demonstrate that engineered strains of S. acidocaldarius DSM639 can produce acetone from CO2 as a carbon source and RISCs as energy sources. The goal is to obtain acetone production under chemolithoautotrophic conditions. (4) Establish, at bioreactor-scale, that engineered S. acidocaldarius DSM639 strains can produce acetone that can be recovered through in situ distillation. T-x-y information indicates that acetone-water mixtures form a two-phase system at aqueous acetone concentrations that will facilitate recovery as a process intensification step. The project will demonstrate the utility of extreme thermoacidophiles as versatile metabolic engineering platforms, presenting a unique approach toward bio-based chemical production from an industrial waste stream.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
硫化合物和二氧化碳作为天然气和石油精炼过程的副产品大量产生,这在其安全处理和经济处置方面产生了重大挑战。幸运的是,微生物可以为开发环保和节能的工艺来回收这些工业废物流提供很多帮助。硫和二氧化碳也恰好是一种叫做嗜热嗜酸古菌的微生物的潜在能量和碳源,这种微生物生长在极热的酸性环境中。处理过量工业硫和二氧化碳的一种潜在方法是设计这些古细菌的代谢途径,使它们利用副产物生产具有商业价值的化学品。该项目将应用最新开发的工具对极端嗜热嗜酸菌进行基因工程改造,以创造新的微生物。这项研究将检验这样一种假设,即将硫氧化基因从一种生物体插入另一种生物体将产生一种能够将二氧化碳和硫转化为工业相关有机化学品的新微生物。将硫氧化基因工程化到在极端条件下自然生长的微生物中具有许多潜在的好处。在热和酸性条件下生长的能力最大限度地减少了不需要的微生物污染的风险,并使生物过程能够像常规化学过程一样操作。微生物的高耐热性也有利于回收具有低沸点的生物化学产品。为了扩大这项研究的教育影响和包容性,北卡罗来纳州聋人学校(NCSD)的教师和学生将通过基于生物技术的教学模块参与。这些单元将包括闭路字幕和其他特点,以促进聋人和听力受损者的教育目标。将在NCSD现场演示模块。此外,NCSU生物技术计划将接待来自NCSD的访问初中和高中学生进行为期一天的实验室体验,这也将使他们熟悉高等教育机会。该项目的目标是对嗜热嗜酸古菌模型Sulfolobus acidocaldarius DSM 639菌株进行代谢工程改造,以从二氧化碳(CO2)和还原无机硫化合物(RISC)中产生化学物质。对极端嗜热嗜酸古菌的泛基因组学分析揭示了一种新的CO2固定循环和关键基因的存在,这些基因可以被招募来使S。酸热菌氧化硫。这些独特的生物学特性将在天然耐热和耐酸生物体的代谢工程中得到利用。代谢工程方法将通过对野生型和重组S.酸热生理学本项目的具体目标是:(1)构建和表征工程S。利用CO2作为碳源和RISC作为能源的嗜酸热菌DSM 639菌株。S. acidocaldarius编码一个CO2固定循环和一个不完整的硫氧化途径,可以用化能自养硫化叶菌的基因修复。(2)证明丙酮的功能性、生物合成、嗜热途径可以工程化到S.酸热菌DSM 639。已经鉴定了可以从其他嗜热微生物中募集的基因/酶,以使S.酸热菌从单糖中生产工业化学品。(3)证明S.酸热菌DSM 639可以从作为碳源的CO2和作为能源的RISC产生丙酮。目标是在化能自养条件下获得丙酮生产。(4)在生物反应器规模上,建立了工程化的S。嗜酸热菌DSM 639菌株可以产生丙酮,其可以通过原位蒸馏回收。T-X-Y信息表明,丙酮-水混合物在含水丙酮浓度下形成两相系统,这将促进作为工艺强化步骤的回收。该项目将展示极端嗜热嗜酸菌作为多功能代谢工程平台的实用性,展示从工业废物流中生产生物基化学品的独特方法。该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Genome Sequences of Five Type Strain Members of the Archaeal Family Sulfolobaceae, Acidianus ambivalens, Acidianus infernus, Stygiolobus azoricus, Sulfuracidifex metallicus, and Sulfurisphaera ohwakuensis
  • DOI:
    10.1128/mra.01490-19
  • 发表时间:
    2020-03-01
  • 期刊:
  • 影响因子:
    0.8
  • 作者:
    Counts, James A.;Vitko, Nicholas P.;Kelly, Robert M.
  • 通讯作者:
    Kelly, Robert M.
Fox Cluster determinants for iron biooxidation in the extremely thermoacidophilic Sulfolobaceae.
  • DOI:
    10.1111/1462-2920.15727
  • 发表时间:
    2022-03
  • 期刊:
  • 影响因子:
    5.1
  • 作者:
    Counts JA;Vitko NP;Kelly RM
  • 通讯作者:
    Kelly RM
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Robert Kelly其他文献

The Impact of Sociodemographic Variables on Functional Recovery Following Lower Extremity Amputation
  • DOI:
    10.1016/j.jvs.2024.06.094
  • 发表时间:
    2024-09-01
  • 期刊:
  • 影响因子:
  • 作者:
    Rylie O'Meara;Karan Chawla;Akshita Gorantla;Robert Kelly;Matthew DeJong;Trissa Babrowski;Pegge Halandras;Matthew Blecha
  • 通讯作者:
    Matthew Blecha
0177 : Deciphering multiple roles of Nkx2-5 during ventricular non-compaction
  • DOI:
    10.1016/s1878-6480(16)30468-2
  • 发表时间:
    2016-04-01
  • 期刊:
  • 影响因子:
  • 作者:
    Caroline Choquet;Minh Nguyen;Frank Kober;Monique Bernard;Robert Kelly;Nathalie Lalevee;Lucile Miquerol
  • 通讯作者:
    Lucile Miquerol
Effect of pH and Al Cations on Chromate Inhibition of Galvanic-Induced Corrosion of AA7050-T7451 Macro-Coupled to 316SS
pH 和 Al 阳离子对 AA7050-T7451 与 316SS 宏观耦合的电偶腐蚀铬酸盐抑制的影响
Kiosk 11R-TA-07 - T1휌 Imaging for Detecting Takotsubo Cardiomyopathy
11R-TA-07 自助服务终端 - T1ρ成像用于检测应激性心肌病
  • DOI:
    10.1016/j.jocmr.2024.100300
  • 发表时间:
    2024-03-01
  • 期刊:
  • 影响因子:
    6.100
  • 作者:
    Liene Balode;James Ross;Dana Dawson;David Gamble;Robert Kelly
  • 通讯作者:
    Robert Kelly
0269: Vascular remodeling of the endocardium following cardiac infarction occured by arteriogenesis and angiogenesis
  • DOI:
    10.1016/s1878-6480(14)71322-9
  • 发表时间:
    2014-04-01
  • 期刊:
  • 影响因子:
  • 作者:
    Lucile Miquerol;Cécile Cassan;Jerome Thireau;Sylvain Richard;Robert Kelly
  • 通讯作者:
    Robert Kelly

Robert Kelly的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Robert Kelly', 18)}}的其他基金

Population Growth and Human Behavioral Change
人口增长和人类行为变化
  • 批准号:
    1939019
  • 财政年份:
    2020
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Populating a Radiocarbon Database of North America, Phase III
填充北美放射性碳数据库,第三阶段
  • 批准号:
    1822033
  • 财政年份:
    2018
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Populating a Radiocarbon Database of North American, Phase II
填充北美放射性碳数据库,第二阶段
  • 批准号:
    1624061
  • 财政年份:
    2016
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Populating a Radiocarbon Database of Western North America
填充北美西部的放射性碳数据库
  • 批准号:
    1418858
  • 财政年份:
    2014
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
COLLABORATIVE RESEARCH: Exploiting microbial hyperthermophilicity to produce an industrial chemical
合作研究:利用微生物的超嗜热性来生产工业化学品
  • 批准号:
    1264052
  • 财政年份:
    2013
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Improvement Grant: Testing Colonization Models in New England
博士论文改进补助金:在新英格兰测试殖民模型
  • 批准号:
    1342656
  • 财政年份:
    2013
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Human Occupation of the Bighorn Mountains
人类对大角山的占领
  • 批准号:
    0710868
  • 财政年份:
    2007
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Functional Approaches for Annotating Secretome-bound Small ORFs in Microbial Genomes
注释微生物基因组中分泌蛋白组结合的小 ORF 的功能方法
  • 批准号:
    0730091
  • 财政年份:
    2007
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Collaborative Research: Biotransformations Near and Above 100°C: Hyperthermophilic Microorganisms and Enzymes for Bioenergy Conversion
合作研究:接近和高于 100°C 的生物转化:用于生物能源转化的超嗜热微生物和酶
  • 批准号:
    0617272
  • 财政年份:
    2006
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
LATE PLEISTOCENE HUMAN OCCUPATION OF THE BIGHORN MOUNTAINS
更新世晚期人类对大角山的占领
  • 批准号:
    0514863
  • 财政年份:
    2005
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant

相似海外基金

The demographic consequences of extreme weather events in Australia
澳大利亚极端天气事件对人口的影响
  • 批准号:
    DP240102733
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Discovery Projects
Attributable impacts from extreme weather events
极端天气事件的影响
  • 批准号:
    NE/Z000203/1
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Research Grant
MCA: Cellular Responses to Thermal Stress in Antarctic Fishes: Dynamic Re-structuring of the Proteome in Extreme Stenotherms
MCA:南极鱼类对热应激的细胞反应:极端钝温鱼蛋白质组的动态重组
  • 批准号:
    2322117
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
RII Track-4:NSF: Improving subseasonal-to-seasonal forecasts of Central Pacific extreme hydrometeorological events and their impacts in Hawaii
RII Track-4:NSF:改进中太平洋极端水文气象事件的次季节到季节预报及其对夏威夷的影响
  • 批准号:
    2327232
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Collaborative Research: Extreme Mechanics of the Human Brain via Integrated In Vivo and Ex Vivo Mechanical Experiments
合作研究:通过体内和离体综合力学实验研究人脑的极限力学
  • 批准号:
    2331294
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Rossbypalooza 2024: A Student-led Summer School on Climate and Extreme Events Conference; Chicago, Illinois; July 22-August 2, 2024
Rossbypalooza 2024:学生主导的气候和极端事件暑期学校会议;
  • 批准号:
    2406927
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Femtosecond X-Ray Diffraction Studies of Crystalline Matter Deforming under Extreme Loading
极端载荷下晶体物质变形的飞秒 X 射线衍射研究
  • 批准号:
    EP/X031624/1
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Research Grant
Collaborative Research: DMREF: Closed-Loop Design of Polymers with Adaptive Networks for Extreme Mechanics
合作研究:DMREF:采用自适应网络进行极限力学的聚合物闭环设计
  • 批准号:
    2413579
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
REU Site: Research Experience for Undergraduates in Resilience Against Extreme Weather Events
REU 网站:本科生抵御极端天气事件的研究经验
  • 批准号:
    2349250
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
Advancing understanding of interannual variability and extreme events in the thermal structure of large lakes under historical and future climate scenarios
增进对历史和未来气候情景下大型湖泊热结构的年际变化和极端事件的了解
  • 批准号:
    2319044
  • 财政年份:
    2024
  • 资助金额:
    $ 30.15万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了