The use of Immunocytochemical Techniques for Phytoplankton Growth Rate Estimation Via Cell Cycle Analysis

9529970泰勒这项研究的主要目标是提出并使用一种易于科学界采用的方法来测量浮游植物的物种特定生长率。该方法遵循了从细胞分裂频率技术到Diel DNA合成法，再到测量生长速度的细胞周期方法的发展。用这种方法，一种保守的细胞周期蛋白，增殖细胞核抗原(增殖细胞核抗原)被用来作为即将分裂的细胞的标志。荧光抗体被用来检测增殖细胞核抗原，标准的荧光显微镜被用来检测抗体的存在或不存在，并识别浮游植物的种类，从而使该方法适用于广泛的研究人员。这项研究是在既定方法的基础上发展起来的，并将使生物海洋学界的很大一部分人受益。浮游生物生物学家的主要目标之一是了解水华爆发的原因以及作为初级生产者的个体物种的贡献。这项研究将建立一种合理、快速、负担得起和坚固耐用的方法来测量物种特定生长率，并将提供关于浮游植物细胞周期蛋白的基本信息。***

9529970 Taylor The primary goal of this research is to advance and use a method, which can easily by employed by the scientific community, for measuring species specific growth rates of phytoplankton. The method follows a progression of developments from the frequency of dividing cells technique, to the diel DNA synthesis method, to the cell cycle approach for measuring growth rates. With this method a conserved cell cycle protein, PCNA (proliferating cell nuclear antigen) is used as a marker of cells which are about to divide. Fluorescent antibodies are used to detect PCNA, and a standard epifluorescence microscope is used for detecting presence or absence of antibodies and identifying species of phytoplankton, thus making the method available to a broad range of investigators. This research builds upon and advances established methodology and will benefit a large segment of the biological oceanographic community. One of the major goals of plankton biologists is to understand the causes of blooms and the contribution of individual species as primary producers. The research will establish a sound, fast affordable and rugged method for measuring species specific growth rates, and will also provide basic information on cell cycle proteins in phytoplankton. ***