Kinetic Studies and Modelling of Interactions of Nutrient, Hormone, Growth Rate and Form Change In Carrot Embryo Development

为了促进对体细胞胚胎发生的理解，首席调查员(PI)提出了一个假设，即胡萝卜胚胎的形态发育与以下因素的综合作用有关营养素，荷尔蒙和几何学。这项拟议的研究的目标是在一个确定的化学环境中通过数学建模来检验这一假设。研究项目的实施将涉及三个方面：-开发定量衡量增长的技术，形式发展，以及正常细胞和温度敏感变异体胚胎培养中的代谢活动。-将假设表述为数学模型，以检验营养物质、生长调节剂、胚胎形态变化的几何构型。-操纵环境因素，以检查它们对胚胎发育的影响。

To advance the understanding of somatic embryogenesis, the Principal Investigators (PIs) have proposed a hypothesis in which form development of the carrot embryo is related to the combined effect of nutrient, hormone and geometry. The objective of the proposed research is to test this hypothesis in a defined chemical environment and via mathematical modeling. The execution of the research project will involve three aspects: - Develop techniques to quantitatively measure growth, form development, and metabolic activities in embryo cultures of normal cells and temperature-sensitive variants. - Formulate the hypothesis as a mathematical model to examine the effect of nutrients, growth regulators, and geometry on form change of embryos. - Manipulate environmental factors to examine their effects on embryogenesis.