Kinetic Studies and Modelling of Interactions of Nutrient, Hormone, Growth Rate and Form Change In Carrot Embryo Development

为了增进对 体细胞胚胎发生 主要研究者（PI） 提出了一个假设， 胡萝卜胚的发育是 与以下因素的综合影响有关 营养素、激素和几何形状。 建议的目标 研究的目的是检验这一假设 在特定的化学环境中 and via通过mathematical数学modeling建模. 的 研究项目的执行 将涉及三个方面： - 开发技术以 定量测量生长， 形式发展，以及 胚胎代谢活动 正常细胞的培养物， 温度敏感 变体。 - 将假设表述为 数学模型检验 营养素的作用， 生长调节剂和 几何形状变化 胚胎 - 操纵环境 因素，以审查其 对胚胎发生的影响。

To advance the understanding of somatic embryogenesis, the Principal Investigators (PIs) have proposed a hypothesis in which form development of the carrot embryo is related to the combined effect of nutrient, hormone and geometry. The objective of the proposed research is to test this hypothesis in a defined chemical environment and via mathematical modeling. The execution of the research project will involve three aspects: - Develop techniques to quantitatively measure growth, form development, and metabolic activities in embryo cultures of normal cells and temperature-sensitive variants. - Formulate the hypothesis as a mathematical model to examine the effect of nutrients, growth regulators, and geometry on form change of embryos. - Manipulate environmental factors to examine their effects on embryogenesis.