Engineering Research Equipment: Confocal Microscope

工程研究设备：共焦显微镜

• 批准号: 9310873
• 负责人: Wei-Shou Hu
• 金额: $ 9.57万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9310873&HistoricalAwards=false
• 关键词: Engineering; Research; Equipment; Confocal; Microscope

9310873 Hu Laser scanning confocal microscopy, with its ability to discriminate against out-of-focus fluorescence and to yield high in-plane resolution, is a powerful tool for three dimensional imaging. Compared to many other analytical tools, such as electron microscopy, it is relatively non-destructive and non-invasive. Biological systems are inherently three-dimensional. The interior of a specimen, ranging from a single cell of a few micrometer thickness to tissues of hundreds of micrometers in depth, can be discerned with a confocal microscope. This Award will provide a confocal microscope to facilitate the following projects: (1) Development of a bioartificial liver; (2) Studies of invasive behavior and cytoskeleton distribution in metastatic cells; (3) Fabrication of a designer path for cell migration in three dimensional gels; (4) Cytoskeletal reorganization during rabbit corneal epithelial cell adhesion; (5) Development of dynamic nutrient feeding strategy for high density mammalian cell bioreactors; and (6) Determination of the partitioning function in cell division.

9310873 Hu激光扫描共聚焦显微镜具有区分离焦荧光和产生高平面内分辨率的能力，是三维成像的有力工具。 与许多其他分析工具（如电子显微镜）相比，它相对非破坏性和非侵入性。 生物系统本质上是三维的。 标本的内部，从几微米厚的单个细胞到几百微米深的组织，都可以用共聚焦显微镜分辨出来。 该奖项将提供共聚焦显微镜，以促进以下项目：（1）生物人工肝的开发;（2）转移细胞的侵袭行为和细胞骨架分布的研究;（3）三维凝胶中细胞迁移的设计路径的制造;（4）兔角膜上皮细胞粘附过程中的细胞骨架重组;（5）生物人工肝的开发。（5）高密度哺乳动物细胞生物反应器的动态营养补料策略的开发;（6）细胞分裂中分配功能的测定。