Engineering Research Equipment: Confocal Microscope

9310873 HU激光扫描共焦显微镜具有对离焦荧光的分辨能力和较高的面内分辨率，是一种强大的三维成像工具。与许多其他分析工具，如电子显微镜相比，它是相对非破坏性和非侵入性的。生物系统本质上是三维的。样品的内部，从几微米厚的单个细胞到数百微米深的组织，都可以用共焦显微镜辨别出来。该奖项将提供共聚焦显微镜，以促进下列项目：(1)生物人工肝的开发；(2)转移细胞侵袭行为和细胞骨架分布的研究；(3)设计细胞在三维凝胶中迁移的路径；(4)兔角膜上皮细胞黏附过程中的细胞骨架重组；(5)为高密度哺乳动物细胞生物反应器开发动态营养喂养策略；以及(6)细胞分裂中分割功能的测定。

9310873 Hu Laser scanning confocal microscopy, with its ability to discriminate against out-of-focus fluorescence and to yield high in-plane resolution, is a powerful tool for three dimensional imaging. Compared to many other analytical tools, such as electron microscopy, it is relatively non-destructive and non-invasive. Biological systems are inherently three-dimensional. The interior of a specimen, ranging from a single cell of a few micrometer thickness to tissues of hundreds of micrometers in depth, can be discerned with a confocal microscope. This Award will provide a confocal microscope to facilitate the following projects: (1) Development of a bioartificial liver; (2) Studies of invasive behavior and cytoskeleton distribution in metastatic cells; (3) Fabrication of a designer path for cell migration in three dimensional gels; (4) Cytoskeletal reorganization during rabbit corneal epithelial cell adhesion; (5) Development of dynamic nutrient feeding strategy for high density mammalian cell bioreactors; and (6) Determination of the partitioning function in cell division.