Acquisition of Radioanalytic Imager

申请资金用于购买BetaScope 603放射分析成像仪，用于检测、记录和分析来自任何一维或二维样品（凝胶、印迹、薄层色谱板等）的b发射同位素（32P、35S、14C）。研究项目包括：1)蛋白质-核酸相互作用的生物物理学；2)真核和原核遗传物质的表达和调控；3)插曲和转座因子生物学；4)真核生物RNA加工的生物学和生物化学；5)核酶的生物化学（即I族自催化内含子）；6)胆固醇和胆汁酸代谢的生物化学和遗传学；7)一类新的MHC相关基因和蛋白产物；8)指导几类终末分化哺乳动物细胞发育的因素。书中还描述了一群研究各种生物学问题的“小用户”。

Funds are requested for an acquisition of the BetaScope 603 Radioanalytic Imager to detect, record and analyze B-emmitting isotopes (32P, 35S, 14C) from any one or two dimensional samples (gels, blots, TLC plates, etc.). Research projects include studies of: 1) the biophysics of protein-nucleic acid interactions; 2) expression and regulation of eukaryotic and prokaryotic genetic material; 3) biology of episomes and transposable elements; 4) the biology and biochemistry of RNA processing in eukaryotes; 5) the biochemistry of ribozymes (i.e., Group I autocatalytic introns); 6) the biochemistry and genetic of cholesterol and bile acid metabolism; 7) a new class of MHC linked genes and protein products; 8) factors directing the development of several classes of terminally differentiated mammalian cell. A group of "minor users" studying a wide range of biological problems is also described.