Radioanalytic Imaging Core Facility

马萨诸塞大学医学中心研究人员的放射分析成像需求已经超出了Betascope印迹分析仪和我们的闪烁计数器的有限能力。研究人员现在需要比现有设施更多的能力来获得高分辨率、定量的放射分析数据。本建议书要求的磷光成像仪设施包括激光扫描仪、高效率、高分辨率存储荧光屏、图像分析工作站、光驱备份系统和灰度级打印机。我们预计，该设施将把我们的放射性分析能力扩大至少四到六倍，以满足目前和未来的需求。除了提高标准分析的速度和效率外，这一独特的存储磷光系统将使我们能够将定性分析转换为定量分析，还将允许我们进行需要对大片区域进行灵敏、高分辨率分析的新颖实验，而这是现有设施无法实现的。

Radioanalytic imaging needs of researchers at the University of Massachusetts Medical Center have overwhelmed the limited capabilities of the Betascope blot analyzer and our scintillation counters. Researchers now require substantially more capacity for obtaining high resolution, quantitative analyses of radioanalytic data than is available with existing facilities. The PhosphorImager facility requested in this proposal includes a laser scanner, high efficiency, high resolution storage phosphor screens, an image analysis work station, optical drive back up system, and a grey scale printer. We anticipate that this facility will expand our capacity for radioanalysis a minimum of four to six fold to meet current and future needs. In addition to increasing the speed and efficiency of standard analyses, this unique storage phosphor system will allow us to convert qualitative assays to quantitative assays and will also permit us to carry out novel experiments requiring sensitive, high resolution analyses of large areas that are impossible with existing facilities.