Multiple Frequency EPR/ENDOR Spectrometer System

要求提供资金，用于最先进的多频 ESP-300 E电子顺磁共振谱仪系统与X波段能力液体 氦氮变温系统，一个变量 温度测角仪和微波计数器。 本研究 设备将用于几个不同的项目。 这些 包括：模式血红素多频EPR研究 复合物，双组氨酸配位的细胞色素B和c，以及 混合金属钴取代血红蛋白的EPR研究 新分离的C型细胞色素和突变型细胞色素C 多频EPR 亚硫酸盐的钼中心和血红素中心的研究 氧化酶和模型钼（V）络合物的EPR研究 光化学或电化学产生的自由基 有机硫化合物及其铜配合物的研究 有机硫化合物的淬灭EPR研究 模型膜结合肽或蛋白质内源荧光 由于模型中脂质上存在自旋标记而产生的残留物 膜，并使用EPR研究的动力学， 模型膜组分的聚合和EPR研究 涉及蛋白质的电子转移过程的动力学- 小分子和蛋白质-蛋白质系统。

Funding is requested for a state-of-the-art multiple frequency ESP-300E EPR spectrometer system with X-band capability liquid helium and nitrogen variable temperature systems, a variable temperature goniometer and a microwave counters. This research equipment will be used for several diverse projects. These include: multiple frequency EPR investigations of model heme complexes, bis-histidine coordinated cytochromes b and c, and mixed metal cobalt-substituted hemoglobins; EPR investigations of newly isolated c-type cytochromes and of mutant cytochromes c produced by site-directed mutagenesis; multiple frequency EPR investigations of the molybdenum and heme centers of sulfite oxidase and model molybdenum (V) complexes; EPR investigations of photochemically or electrochemically produced radicals of organosulfur compounds and investigations of copper complexes of organosulfur compounds; EPR studies of the quenching of the intrinsic fluorescence of model membrane-bound peptide or protein residue due to the presence of spin labels on lipids in the model membranes, and the use of EPR to investigate the kinetics of polymerization of model membrane component; and EPR investigation of the kinetics of electron transfer processes involving protein- small molecular and protein-protein systems.