Polymerized Bicontinous Cubic Lipid Assemblies

小行星9523297奥布莱恩 近年来，由于对非层状结构可能的生物学功能的兴趣，生物和模型脂质膜中非层状脂质结构的形成已被广泛研究。 在一定的温度和浓度条件下，极性脂质形成几何复杂的液晶结构。 这些非层状相包括双连续立方相（QII）和倒置六方相（HII）。 无论非层状相的生物学意义如何，很明显，稳定的非层状结构提供了新的技术材料。在我们对非层状相聚合的头两年研究中，我们已经成功地证明了一种稳定它们的策略。 继续研究的目的是利用这种经过验证的策略，以保留脂质和水相网络并保留其界面的生物相容性的方式制备稳定的QII和HII相家族。 为了实现这些目标的研究将涉及新的可聚合脂质的设计和合成，水合未聚合的脂质组件的相行为的表征，以及在非层状相的脂质的受控聚合，仔细注意聚合物的性质，即聚合物链的位置和大小，以及聚合物交联的程度。 然后将确定聚合的非层状组装体的相行为和稳定性。 非层状相将以这样的方式稳定，即扩展有利于所需非层状结构存在的温度和浓度范围，以及增加组装体对表面活性剂或有机溶剂溶解的抗性。 这项研究将为研究人员提供新的生物材料，以探测脂水组件的物理和化学。 由于已知几种QII相在对称性和水性通道的尺寸方面不同，因此可聚合脂质将被设计成形成每种常见基团的QII相，以产生一族稳定的有机沸石。 QII相的互穿网络表明这些有机沸石的潜在应用，其充分利用脂质膜表面用于试剂的定位、抗体片段或抗体酶的固定化、或用于化学或生物转化、诊断和/或分离应用的蛋白质的掺入。 聚合的HII相预期在高度期望的情况下提供具有均匀孔径的规则六边形阵列。3至8 nm直径范围，即大到足以允许球状蛋白或水溶性无规卷曲聚合物流动。 该研究为非层状组装体的技术应用提供了科学依据。 该研究将寻求对聚合物链的形成、位置和性质对非层状相特征的影响提供连贯的理解。 ***

9523297 O'Brien The formation of nonlamellar lipid structures in biological and model lipid membranes has been extensively investigated in recent years, because of the interest in the possible biological function of nonlamellar structures. At certain conditions of temperature and concentration polar lipids form geometrically complex liquid crystalline structures. These nonlamellar phases include bicontinuous cubic phases (QII) and the inverted hexagonal phase (HII). Whatever may be the biological significance of nonlamellar phases, it is clear that stabilized nonlamellar structures offer novel technological materials, In the first two years of our research into the polymerization of nonlamellar phases we have successfully demonstrated a strategy for their stabilization. The objective then of the continuing research is to utilize this proven strategy to prepare a family of stabilized QII as well as HII phases in a manner that preserves both the lipid and aqueous phase networks and retains the biocompatibility of their interface. In order to accomplish these goals the research will involve the design and synthesis of new polymerizable lipids, the characterization of the phase behavior of the hydrated unpolymerized lipid assemblies, and the controlled polymerization of the lipids in nonlamellar phases with careful attention to the nature of the polymer, i.e. the location and size of the polymer chains, and the extent of polymer crosslinking. The phase behavior and stability of the polymerized nonlamellar assemblies will then be determined. The nonlamellar phases will be stabilized in a manner that extends the range of temperatures and concentrations that favor the existence of the desired nonlamellar structure, as well as increases the resistance of the assembly to dissolution by surfactants or organic solvents. This research will provide researchers with novel biomaterials to probe the physics and chemistry of lipid-water assemblies. Since several QII phases are known that vary in sy mmetry and the size of the aqueous channels, therefore polymerizable lipids will be designed to form QII phases of each of the commonly found groups in order to generate a family of stabilized organic zeolites. The interpenetrating networks of the QII phase suggests potential applications of these organic zeolites, which take full advantage of the lipid membrane surface for the localization of reagents, the immobilization of antibody fragments or abenzymes, or the incorporation of proteins for chemical or biological conversions, diagnostics, and/or separation applications. Polymerized HII phases are expected to provide regular hexagonal arrays with uniform pore sizes in the highly desirable ca. 3 to 8 nm diameter range, i.e. large enough to permit the flow of globular proteins or water soluble random coil polymers. The proposed research will provide a scientific basis for the technological utilization of nonlamellar assemblies. The research will seek to provide a coherent understanding of the effect of the formation, location, and nature of polymer chains on the characteristics of nonlamellar phases. ***