Diffusion and Binding in Polymerized Bicontinuous Cubic Phases

abstractcts - 9816826大卫O 'BrienU。天然和合成极性脂类可以形成多种水合非层状相，包括倒六边形（HII）和倒双连续立方（QII）相。容易形成一种或多种这些非层状相的脂质有时被称为多态脂质。适当设计可聚合的多态脂质可以成功地形成这些非层状相，并通过交联聚合使其稳定。双连续的立方形态由弯曲的脂质双分子层组成，分离了相互渗透的水通道。首席研究员介绍了一种成功的非层状相聚合策略，该策略保留了这些非层状相的形态，并以一种提高温度范围和耐有机溶剂性的方式稳定了组装。因此，脂质结构域可以转化为具有广泛聚合物性质的聚（脂质），同时使水结构域不受干扰，以便进一步细化。聚合的QII相的互穿网络表明了这些有机沸石的潜在应用，它们充分利用脂质膜表面来定位试剂，固定抗体片段或抗酶，或结合蛋白质进行化学或生物转化，诊断和/或分离应用。本提案的目的是表征分子大小对水溶性大分子在聚合QII相的水通道内扩散的影响。大分子扩散将实验确定使用脉冲场梯度核磁共振技术。形成和稳定QII相的材料将来自易于合成的可聚合脂类家族。这是一年的临终补助金。

ABSTRACTCTS-9816826David O'BrienU. of ArizonaNatural and synthetic polar lipids can form a variety of hydrated nonlamellar phases, including the inverted hexagon (HII) and inverted bicontinuous cubic (QII) phases. Lipids that readily form one or more of these nonlamellar phases are sometimes termed polymorphic lipids. Appropriately designed polymerizable polymorphic lipids can be successfully used to form either of these nonlamellar phases and stabilize them via crosslinking polymerization. The bicontinuous cubic morphologies consist of curved lipid bilayers that separate interpentrating water channels. The principal investigator's has introduced a successful strategy for the polymerization of these nonlamellar phases, that retains their morphology and stabilizes the assembly in a manner that enhances both the temperature range and resistance to organic solvents. Thus the lipid domains can be converted to poly(lipid) with a wide range of polymer properties, while leaving the water domains unperturbed for further elaboration. The interpentrating networks of the polymerized QII phases suggests potential applications of these organic zeolites, which take full advantage of the lipid membrane surface for the localization of reagents, the immobilization of antibody fragments or abenzymes, or the incorporation of proteins for chemcial or biological conversions, diagnostic, and/or separation applications. The objective of this proposal is to characterize the effect of molecular size on the diffusion of water-soluble macromolecules within the water channels of polymerized QII phases. Macromolecular diffusion will be experimentally determined using pulsed field gradient NMR techniques. The materials for the formation and stabilization of QII phases will come from a family of readily synthesized polymerizable lipids. This is a terminal grant for one year.