Diffusion and Binding in Polymerized Bicontinuous Cubic Phases

摘要CTS-9816826 David O 'BrienU.天然和合成极性脂质可以形成各种水合非层状相，包括倒置六边形（HII）和倒置双连续立方（QII）相。 容易形成一个或多个这些非层状相的脂质有时被称为多晶型脂质。 适当设计的可聚合多晶型脂质可以成功地用于形成这些非层状相中的任一种，并通过交联聚合来稳定它们。 双连续立方形态由弯曲的脂质双层组成，这些脂质双层分隔相互渗透的水通道。 主要研究者已经引入了一种成功的策略，用于这些非层状相的聚合，该策略保留了它们的形态并以增强温度范围和对有机溶剂的耐受性的方式稳定了组装。 因此，脂质结构域可以转化为具有广泛聚合物性质的聚（脂质），同时使水结构域不受干扰以进行进一步加工。 聚合的QII相的互穿网络表明这些有机沸石的潜在应用，其充分利用脂质膜表面用于试剂的定位、抗体片段或抗体酶的固定化、或用于化学或生物转化、诊断和/或分离应用的蛋白质的掺入。 本提案的目的是表征聚合的QII相的水通道内的水溶性大分子的扩散的分子尺寸的效果。 将使用脉冲场梯度NMR技术通过实验确定大分子扩散。 用于形成和稳定QII相的材料将来自易于合成的可聚合脂质家族。 这是为期一年的终止补助金。

ABSTRACTCTS-9816826David O'BrienU. of ArizonaNatural and synthetic polar lipids can form a variety of hydrated nonlamellar phases, including the inverted hexagon (HII) and inverted bicontinuous cubic (QII) phases. Lipids that readily form one or more of these nonlamellar phases are sometimes termed polymorphic lipids. Appropriately designed polymerizable polymorphic lipids can be successfully used to form either of these nonlamellar phases and stabilize them via crosslinking polymerization. The bicontinuous cubic morphologies consist of curved lipid bilayers that separate interpentrating water channels. The principal investigator's has introduced a successful strategy for the polymerization of these nonlamellar phases, that retains their morphology and stabilizes the assembly in a manner that enhances both the temperature range and resistance to organic solvents. Thus the lipid domains can be converted to poly(lipid) with a wide range of polymer properties, while leaving the water domains unperturbed for further elaboration. The interpentrating networks of the polymerized QII phases suggests potential applications of these organic zeolites, which take full advantage of the lipid membrane surface for the localization of reagents, the immobilization of antibody fragments or abenzymes, or the incorporation of proteins for chemcial or biological conversions, diagnostic, and/or separation applications. The objective of this proposal is to characterize the effect of molecular size on the diffusion of water-soluble macromolecules within the water channels of polymerized QII phases. Macromolecular diffusion will be experimentally determined using pulsed field gradient NMR techniques. The materials for the formation and stabilization of QII phases will come from a family of readily synthesized polymerizable lipids. This is a terminal grant for one year.