Acquisition of 300MHz NMR Spectrometer

购置 300MHz 核磁共振波谱仪

• 批准号: 9016484
• 负责人: Gerd La Mar
• 金额: $ 20.5万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9016484&HistoricalAwards=false
• 关键词: Acquisition; 300MHz; NMR; Spectrometer

This Proposal requests partial funding for a vertical bore, intermediate field NMR spectrometer for high resolution spectroscopy in structural biology. The instrument will consist of a 7.05 T magnet, sufficient homogeneous volume for 10 mm proton and 20 mm heteronuclei sample tubes, and a console with all features necessary for advanced techniques such as proton detection of heteronuclei, spin locking, multiple quantum experiments, and all other modern two dimensional methods. The spectrometer will be utilized by a core group of five Major User (four with NSF funding) and several minor users. The projects of the Major Users focus on NMR applications to structural biology. Planned research using the proposed spectrometer emphasizes studies of biomolecule structure and model compounds of protein active sites, primarily on systems containing paramagnetic metal centers. Each investigator has an established program of NMR research with spectrometers of lesser capability, and the Proposal documents the additional knowledge to be gained by the new capabilities of the proposed instrument. The proposed instrument will come under the auspices of the UC Davis NMR Facility. The Facility has a proven record of over ten years in implementing state-of-the-art NMR techniques, and in spectrometer maintenance and modification.

本提案要求为结构生物学中用于高分辨率光谱的垂直孔、中间场核磁共振光谱仪提供部分资金。该仪器将包括一个7.05 T的磁铁，足够容纳10毫米质子和20毫米异核样管的均匀体积，以及一个具有先进技术所需的所有功能的控制台，如异核质子检测，自旋锁定，多重量子实验，以及所有其他现代二维方法。该光谱仪将由5个主要用户（其中4个由国家科学基金会资助）和几个次要用户组成的核心小组使用。主要用户的项目集中在核磁共振在结构生物学中的应用。计划使用该光谱仪的研究重点是研究生物分子结构和蛋白质活性位点的模型化合物，主要是含有顺磁性金属中心的系统。每个研究人员都有一个既定的核磁共振研究计划，使用性能较低的光谱仪，并且提案记录了通过拟议仪器的新功能获得的额外知识。提议的仪器将由加州大学戴维斯分校核磁共振设施赞助。该设施在实施最先进的核磁共振技术以及光谱仪维护和修改方面拥有超过十年的成熟记录。