Acquisition of 300MHz NMR Spectrometer

这项提案要求部分资金用于结构生物学中高分辨率光谱学的垂直钻孔、中场核磁共振光谱仪。该仪器将包括一个7.05T磁体，足够容纳10毫米质子和20毫米异核样品管的均匀体积，以及一个控制台，具有先进技术所需的所有功能，如质子探测异核、自旋锁定、多量子实验和所有其他现代二维方法。光谱仪将由五个主要用户(四个由NSF资助)和几个次要用户组成的核心小组使用。主要用户的项目集中在核磁共振在结构生物学中的应用。使用建议的光谱仪计划进行的研究侧重于蛋白质活性中心的生物分子结构和模型化合物的研究，主要是包含顺磁金属中心的系统。每个研究人员都有一个用较小能力的光谱仪进行核磁共振研究的既定计划，该提案记录了通过拟议仪器的新功能将获得的额外知识。拟议的仪器将由加州大学戴维斯分校核磁共振设施赞助。该设施在实施最先进的核磁共振技术以及光谱仪维护和改造方面拥有十多年的成熟记录。

This Proposal requests partial funding for a vertical bore, intermediate field NMR spectrometer for high resolution spectroscopy in structural biology. The instrument will consist of a 7.05 T magnet, sufficient homogeneous volume for 10 mm proton and 20 mm heteronuclei sample tubes, and a console with all features necessary for advanced techniques such as proton detection of heteronuclei, spin locking, multiple quantum experiments, and all other modern two dimensional methods. The spectrometer will be utilized by a core group of five Major User (four with NSF funding) and several minor users. The projects of the Major Users focus on NMR applications to structural biology. Planned research using the proposed spectrometer emphasizes studies of biomolecule structure and model compounds of protein active sites, primarily on systems containing paramagnetic metal centers. Each investigator has an established program of NMR research with spectrometers of lesser capability, and the Proposal documents the additional knowledge to be gained by the new capabilities of the proposed instrument. The proposed instrument will come under the auspices of the UC Davis NMR Facility. The Facility has a proven record of over ten years in implementing state-of-the-art NMR techniques, and in spectrometer maintenance and modification.