Ion Transport in Freshwater Mussels: Ion Specific Channels

淡水贻贝中的离子传输：离子特定通道

• 批准号: 9017461
• 负责人: Thomas Dietz
• 金额: $ 24.8万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9017461&HistoricalAwards=false
• 关键词: Ion; Transport; Freshwater; Mussels; Specific

Freshwater mussels are successful ionic- and osmotic-regulators but they maintain blood ion concentrations well below other freshwater animals. Using various procedures including ion fluxes, ion substitution studies, isolated tissue techniques, morphology and histochemistry, cAMP and prostaglandin biochemistry, we have demonstrated that the gill is the a major site of ionic regulation. We have found that freshwater mussels transport Na, K, Rb, and Cl at rates that equal or exceed the transport rates found inmost freshwater forces involved in ion transport, the electrical gradient across gill epithelial cells must be measured. In addition, the types and their respective conductances of ion channels present in epithelial cell membranes will be determined using the inside-out patch clamp technique. Preliminary data indicate that mussel membranes have ion channels with remarkably high (300 pS) conductances. These studies will provide information about how mussels function with low blood ionic strengths, and yet tolerate substantial modification of blood ion composition when subjected to changes in their environment. Understanding the cellular mechanisms that allow freshwater mussels such remarkable adaptations is relevant to our understanding of the limits at which cells and organisms can function. //

淡水贻贝是成功的离子和非离子调节剂， 它们的血液离子浓度远低于其他淡水 动物 使用各种程序，包括离子通量，离子 替代研究、分离组织技术、形态学和 组织化学、cAMP和前列腺素生物化学，我们有 表明鳃是离子调节的主要部位。 我们已经发现淡水贻贝运输Na、K、Rb和Cl 其速率等于或超过了在大多数 淡水力量参与离子运输，电 必须测量跨鳃上皮细胞的梯度。 在 此外，离子的类型和它们各自的电导 将确定上皮细胞膜中存在的通道 使用由内而外的膜片钳技术。 初步数据 表明贻贝膜具有显著 高电导（300 pS）。 这些研究将提供 关于贻贝如何在低血液离子 强度，但仍耐受血液离子的实质性改变 当他们的环境发生变化时。 了解淡水贻贝的细胞机制 这种显著的适应性与我们对 细胞和生物体能够发挥作用的极限。 //