Regulation of Neurohormone Synthesis in Insects

这项拟议中的研究将检查神经激素的合成， 分泌在昆虫使用hypertrehalosemic激素（HTH）作为 该模型 HTH属于脂肪运动/红色素- 在昆虫中广泛分布的浓缩激素家族。 这些激素都刺激动员脂肪体营养 以提升循环代谢物的能量。HTH也 在脂肪体线粒体成熟期间刺激血红素合成 并与保幼激素协同作用， 合成. 因此，HTH是调节昆虫基础代谢的关键。 通过影响能量产生/消耗来进行新陈代谢。 HTH合成-分泌的测量将通过孵育 心体有三个H!色氨酸，并确定 我!使用反相色谱法和闪烁法形成的HTH 光谱法 这项研究将检查HTH的合成-分泌 相对于一系列生理变量，包括：年龄， 性别、昼夜节律、喂养、内分泌滴度和 神经系统的其他部分。 这项研究将提供一个 为未来研究生理学如何 效应子控制高等动物神经激素基因的表达。 生理变化对环境变化的反应 动物是由神经系统介导的，反过来， 神经激素，肽类激素， 神经系统 本提案描述了对一个这样的问题的研究， 神经激素，蟑螂的高海藻酸盐激素（HTH） Blaberus discoidalis，一种参与动员的激素 从昆虫的脂肪体中储存能量。 该项目将 分离编码HTH的基因，并将研究其生理特性。 在基因水平上调节HTH合成。 结果应 提供有关神经激素在以下方面作用的基本信息： 控制对环境刺激的生理反应。 等 基本信息在未来的应用中也可能是有用的， 防治病虫害的方法//

The proposed research will examine neurohormone synthesis and secretion in insects using the hypertrehalosemic hormone (HTH) as the model. HTH belongs to the adipokinetic/red pigment- concentrating hormone family that is widespread among insects. These hormones all stimulate mobilization of fat body nutrient stores to elevate circulating metabolites for energy. HTH also stimulates heme synthesis during fat body mitochondrial maturation and acts synergistically with juvenile hormone to enhance protein synthesis. Hence, HTH is critical for regulating insect basal metabolism through effects on energy production/consumption. Measurements of HTH synthesis-secretion will be made by incubating corpora cardiaca with ı3H!tryptophan and determine the amount of ı3H!HTH formed using reversed-phase chromatography and scintillation spectrometry. The research will examine HTH synthesis-secretion relative to a series of physiological variables that include: age, sex, circadian rhythms, feeding, endocrine titers and effects by other parts of the nervous system. This research will provide a base of data for future investigations into how physiological effectors control neurohormone gene expression in higher animals. Physiological changes in response to environmental changes in animals are mediated by the nervous system and, in turn, by neurohormones, peptide hormones that are produced and secreted by the nervous system. This proposal describes studies on one such neurohormone, the hypertrehalosemic hormone (HTH) of the cockroach Blaberus discoidalis, a hormone involved in the mobilization of energy reserves from the fat body of the insect. The project will isolate the gene encoding HTH, and will study the physiological regulation of HTH synthesis at the gene level. The results should provide basic information about the role of neurohormones in controlling physiological responses to environmental stimuli. Such basic information may also be useful in future applications in the area of insect pest control.//