Transposition of Bacteriophage Mu DNA

噬菌体 Mu DNA 转座

• 批准号: 9018328
• 负责人: Martin Pato
• 金额: $ 28.5万
• 依托单位: University of Colorado at Denver
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018328&HistoricalAwards=false
• 关键词: Transposition; Bacteriophage; Mu; DNA

Our objective is understanding molecular mechanisms of transposition of mobiles genetic elements. We study bacteriophage Mu, as mechanisms used in Mu transposition are highly analogous to those of other mobile genetic elements, prokaryotic and eukaryotic. Thus unique properties of Mu as a lysogenic virus and a transposon allow use of in vivo biochemical studies which are difficult or impossible in systems with low frequency of transposition. During the present grant period, we will focus on understanding the role of a novel transposition enhancer (CEN), located in the center of the Mu genome, which contains a strong DNA gyrase binding site. We will test models for use of CEN-like enhancers in other systems. CEN is the clearest example presently available of a gyrase binding site with a specific function. Study of its mechanism of action should help in understanding not only the role of gyrase in transposition, but also in organization of large DNA structures such as the prokaryotes nucleoid.

我们的目标是了解 移动体遗传元件的转座。 我们研究噬菌体 Mu，因为Mu转座中使用的机制高度类似于 其他移动的遗传元件，原核生物和真核生物。 因此，Mu作为溶原性病毒和转座子的独特性质 允许使用体内生物化学研究，这是困难的，或 不可能在低频率的换位系统中。 期间 在目前的资助期内，我们将重点了解 一种新的转座增强子（CEN），位于 Mu基因组，其中含有一个强的DNA促旋酶结合位点。 我们将测试在其他系统中使用CEN样增强子的模型。 CEN是目前可获得的关于促旋酶结合的最清楚的例子。 具有特定功能的网站。 作用机制研究 不仅有助于理解促旋酶在 转座，但也在组织大的DNA结构 如原核生物的类核。