STUDIES ON BACTERIOPHAGE MU DNA TRANSPOSITION

噬菌体MU DNA转座的研究

• 批准号: 3282705
• 负责人: Rasika M Harshey
• 金额: $ 21.01万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3282705
• 关键词: DNA binding protein; DNA replication; Escherichia coli; bacterial proteins; bacterial virus; chromosome deletion; chromosome translocation; gene mutation; genetic manipulation; genetic mapping; laboratory mouse; laboratory rabbit; nucleic acid sequence; plasmids; posttranscriptional RNA processing; protein structure; transposon /insertion element; virus DNA

Transposable genetic elements are the generators of a variety of rearrangements which mutate genes and perturb their regulation. These elements promote and regulate their own movement. Their study is important therefore in understanding how the proteins they encode interact with DNA to modify it both by recombining and regulating its genetic potential. Our studies will focus on the model transponson, phage Mu. Mu is unique in being the only transposon whose transposition proteins function efficiently in vitro and are therefore amenable to elaborate biochemical analysis. Mu encodes two mechanisms of DNA transposition- conservative and replicative DNA transposition. We propose to carry out a detailed study of the phage proteins A and B required for both mechanisms of Mu transposition, with a view to understanding structure-function relationships in thes proteins. This would help us understand the molecular details of DNA- protein and protein-protein interactions fundamental to transposition as well as to DNA excision. We also propose to study the 64kDa protein found in infecting Mu DNA. These studies will be important in elucidating the mechanism of conservative DNA transposition. Mu DNA transposition is regulated at many levels. We propose experiments that specifically address the post-transcriptional regulation of A- protein synthesis. We hope to uncover novel ways in which transposases limit their expression and thereby their mutagenic potential.

转座基因元件是多种转座基因的发生器。 基因重排使基因突变并扰乱其调节。 这些元素促进和调节自己的运动。 他们的 因此，研究对于理解蛋白质 它们编码与DNA相互作用，通过重组 并调节其遗传潜能。 我们的研究将集中在 模式转座子，噬菌体Mu。 穆是独一无二的， 转座子，其转座蛋白在 因此可以进行精细的生化处理 分析. Mu编码两种DNA转座机制- 保守性和复制性DNA转座。 我们建议 对所需的噬菌体蛋白A和B进行详细研究 对于Mu转位的两种机制， 了解这些蛋白质的结构与功能关系。 这将帮助我们了解DNA的分子细节- 蛋白质和蛋白质-蛋白质相互作用 转座以及DNA切除。 我们亦建议 研究在感染Mu DNA中发现的64 kDa蛋白。 这些 研究将是重要的阐明机制， 保守DNA转座 Mu DNA转座是 在多个层面进行监管。 我们提出的实验， 具体解决转录后调节A- 蛋白质合成。 我们希望能发现新的方法， 转座酶限制了它们的表达，从而限制了它们的诱变性。 潜力