BACTERIOPHAGE MU DNA TRANSPOSITION

噬菌体 MU DNA 转座

• 批准号: 3282707
• 负责人: Rasika M Harshey
• 金额: $ 20.93万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3282707
• 关键词: DNA binding protein; DNA replication; Escherichia coli; bacterial proteins; bacterial virus; chromosome deletion; chromosome translocation; gene mutation; genetic manipulation; genetic mapping; laboratory mouse; laboratory rabbit; nucleic acid sequence; plasmids; protein structure; transposon /insertion element; virus DNA

Transposable genetic elements are the generators of a variety of rearrangements which mutate genes and perturb their regulation. These elements promote and regulate their own movement. Their study is important therefore in understanding how the proteins they encode interact with DNA to modify it both by recombining and regulating its genetic potential. Our studies will focus on the model transponson, phage Mu. Mu is unique in being the only transposon whose transposition proteins function efficiently in vitro and are therefore amenable to elaborate biochemical analysis. Mu encodes two mechanisms of DNA transposition- conservative and replicative DNA transposition. We propose to carry out a detailed study of the phage proteins A and B required for both mechanisms of Mu transposition, with a view to understanding structure-function relationships in thes proteins. This would help us understand the molecular details of DNA- protein and protein-protein interactions fundamental to transposition as well as to DNA excision. We also propose to study the 64kDa protein found in infecting Mu DNA. These studies will be important in elucidating the mechanism of conservative DNA transposition. Mu DNA transposition is regulated at many levels. We propose experiments that specifically address the post-transcriptional regulation of A- protein synthesis. We hope to uncover novel ways in which transposases limit their expression and thereby their mutagenic potential.

转座遗传元件是多种基因的产生者 使基因突变并扰乱其调节的重排。 这些元素促进并调节它们自身的运动。 他们的 因此，研究对于理解蛋白质如何 它们编码与 DNA 的相互作用，通过重组来修改 DNA 并调节其遗传潜力。 我们的研究将集中于 模型转座子，噬菌体Mu。 Mu 是独一无二的 转座子，其转座蛋白在 体外，因此适合详细阐述生化 分析。 Mu 编码 DNA 转座的两种机制 - 保守性和复制性 DNA 转座。 我们建议 对所需的噬菌体蛋白 A 和 B 进行详细研究 对于 Mu 转座的两种机制，以期 了解这些蛋白质的结构与功能关系。 这将有助于我们了解 DNA 的分子细节—— 蛋白质和蛋白质-蛋白质相互作用的基础 转座以及 DNA 切除。 我们还建议 研究感染 Mu DNA 中发现的 64kDa 蛋白质。 这些 研究对于阐明其机制具有重要意义 保守的DNA转座。 Mu DNA 转座是 受到多个层面的监管。 我们建议进行实验 专门解决A-的转录后调控 蛋白质合成。 我们希望发现新颖的方式 转座酶限制了它们的表达，从而限制了它们的诱变性 潜在的。