BACTERIOPHAGE MU DNA TRANSPOSITION

可转座的遗传元件是各种 基因突变并扰乱基因调控的重排。 这些因素促进和调节着它们自身的运动。他们的 因此，研究对于理解蛋白质是如何 它们通过编码与DNA相互作用，通过重组来修改两者 并调节其遗传潜力。我们的研究将集中在 模型应答，噬菌体Mu。Mu的独特之处在于它是唯一的 转座子的转座蛋白在 在体外，因此可以进行精细的生物化学 分析。MU编码两种DNA转座机制- 保守的和复制的DNA转位。我们建议 对所需的噬菌体蛋白A和B进行详细研究 对于Mu移位的两种机制，以期 了解这些蛋白质中的结构-功能关系。 这将帮助我们了解DNA的分子细节- 蛋白质和蛋白质-蛋白质相互作用是 转位以及DNA切除。我们还建议 研究在感染Mu DNA中发现的64 kDa蛋白。这些 研究对于阐明其发病机制具有重要意义。 保守的DNA转位。MU DNA转座是 在多个层面上进行监管。我们建议进行一些实验， 具体解决A-A转录后调控- 蛋白质合成。我们希望发现一些新的方式， 转座酶限制了它们的表达，从而限制了它们的突变性 潜力。

Transposable genetic elements are the generators of a variety of rearrangements which mutate genes and perturb their regulation. These elements promote and regulate their own movement. Their study is important therefore in understanding how the proteins they encode interact with DNA to modify it both by recombining and regulating its genetic potential. Our studies will focus on the model transponson, phage Mu. Mu is unique in being the only transposon whose transposition proteins function efficiently in vitro and are therefore amenable to elaborate biochemical analysis. Mu encodes two mechanisms of DNA transposition- conservative and replicative DNA transposition. We propose to carry out a detailed study of the phage proteins A and B required for both mechanisms of Mu transposition, with a view to understanding structure-function relationships in thes proteins. This would help us understand the molecular details of DNA- protein and protein-protein interactions fundamental to transposition as well as to DNA excision. We also propose to study the 64kDa protein found in infecting Mu DNA. These studies will be important in elucidating the mechanism of conservative DNA transposition. Mu DNA transposition is regulated at many levels. We propose experiments that specifically address the post-transcriptional regulation of A- protein synthesis. We hope to uncover novel ways in which transposases limit their expression and thereby their mutagenic potential.