Molecular Studies on the Biogenesis of Polyhydroxybutyrate and Related Polyesters

聚羟基丁酸酯及相关聚酯生物合成的分子研究

• 批准号: 9018546
• 负责人: Joanne Stubbe
• 金额: $ 32.2万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-15 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018546&HistoricalAwards=false
• 关键词: Molecular; Studies; Biogenesis; Polyhydroxybutyrate; Related

The potential for utilizing biological systems as a source of biodegradable thermoplastics is becoming increasingly attractive in view of the environmental problems associated with the disposal of oil based polymers. A. eutrophus H16 and P. oleovorans are biological organisms that produce poly D-3 hydroxyalkanoates (PHAs) with interesting polymeric properties. The genes for the polymerases from these organisms have been cloned and sequenced. The present proposal seeks support to develop methods for high level expression of the polymerases. Large amounts of protein should facilitate isolation of polymerases, characterization of their substrate specificities and study of detailed reaction mechanism. A detailed understanding of biological polyester formation should allow generation of PHA analogs (polymers and copolymers) of defined composition, length and functionality.

鉴于与处置油基聚合物有关的环境问题，利用生物系统作为可生物降解热塑性塑料来源的潜力正变得越来越有吸引力。A.富营养菌H16和P. oleovorans是产生具有有趣聚合特性的聚D-3羟基烷烃酸酯（PHAs）的生物有机体。这些生物体的聚合酶基因已被克隆并测序。目前的建议寻求支持开发方法的高水平表达的聚合酶。大量的蛋白质有助于分离聚合酶，表征其底物特异性和研究详细的反应机制。对生物聚酯形成的详细了解应该允许生成具有确定组成、长度和功能的PHA类似物（聚合物和共聚物）。