Equipment for a Labortory Course in Biological Chemistry

生物化学实验课程设备

• 批准号: 9351732
• 负责人: Joanne Stubbe
• 金额: $ 5.67万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-01 至 1995-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9351732&HistoricalAwards=false
• 关键词: Equipment; Labortory; Course; Biological; Chemistry

The project involves development of a Biochemistry course within the Chemistry Department. The course will introduce will ultimately be composed of five modules which will include: l) purification of >-galactosidase from Ecoli; 2) cloning of the gene for this protein; 3) preparation of site directed mutants to investigate the mechanism of this protein; 4) use of steady state and presteady state kinetics as probes of the mechanism of this enzyme. The fifth module will be developed by each student with the assistance and input from the instructor.

该项目涉及生物化学课程的发展， 化学系。 本课程将介绍威尔 最终将由五个模块组成，包括： 从大肠杆菌中纯化β-半乳糖苷酶; 2）基因的克隆 3）制备定点突变体， 探讨该蛋白的作用机制; 4）利用稳态 和稳态前动力学作为这一机制的探针 酵素第五个模块将由每个学生开发， 指导员的协助和输入。