Cellular Mg2+ Regulation: Role of ATP and Growth Factors

细胞 Mg2 调节：ATP 和生长因子的作用

• 批准号: 9018677
• 负责人: Robert Grubbs
• 金额: $ 24万
• 依托单位: Wright State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018677&HistoricalAwards=false
• 关键词: Cellular; Mg2+; Regulation; Role; ATP

While it is clear that cells maintain a cytoplasmic free magnesium level well below the magnesium equilibrium potential and require magnesium ion for the normal activity of many enzymes, the role of intracellular magnesium in regulatory events in cellular physiology remains largely unexplored. Attempts to study mechanisms that regulate intracellular magnesium levels and to assess the physiological role of this cation have been limited by the lack of adequate techniques for measurement of intracellular free magnesium. The development of magnesium-sensitive fluorescent indicators that can be loaded into cells has made it possible to monitor intracellular magnesium on a second by second basis for the first time. Having established a technique for utilizing one of these new indicators, Mag-fura2, to measure internal magnesium in BC3H-1 cells, the primary goals of this proposal are: 1) to characterize the kinetic relationship between intracellular magnesium and ATP/creatine phosphate levels in BC3H-1 cells, 2) to develop a technique for determining the magnesium buffer capacity of these cells, and 3) to characterize the effect of acute exposure to epidermal growth factor and other growth factors on ATP levels in BC3H-1 and Swiss 3T3 cells. Since cells maintain their intracellular magnesium levels within certain limits, and require magnesium for the regulation of a variety of critical cellular functions, it seems likely that regulation of intracellular magnesium is a physiologically important process. Recent advances in the development of fluorescent probes for magnesium ion now make it possible to approach this problem. The proposed research will take advantage of these new research tools to provide fundamental new information about the intracellular regulation of magnesium, the most abundant cation found in mammalian cells.

虽然很明显，细胞维持细胞质游离镁 水平远低于镁平衡电位，需要 镁离子对于许多酶的正常活性， 细胞内镁在细胞生理调节中作用 大部分尚未开发。 试图研究的机制， 调节细胞内镁水平，并评估 这种阳离子的生理作用受到缺乏 测量细胞内游离 镁。 镁敏荧光染料的研究进展 可以加载到细胞中的指标使得有可能 监测细胞内镁在第二次由第二个基础上， 首次 建立了一种技术， 这些新的指标，Mag-fura 2，测量内部镁， BC 3 H-1细胞，该提议的主要目标是：1） 表征细胞内 BC 3 H-1细胞中的镁和ATP/磷酸肌酸水平，2）至 开发一种测定镁缓冲容量的技术 这些细胞，和3）表征急性暴露的影响 表皮生长因子和其他生长因子对ATP水平的影响 在BC 3 H-1和Swiss 3 T3细胞中。 由于细胞维持其细胞内镁水平， 一定的限制，并需要镁的调节， 各种关键的细胞功能，似乎很可能， 细胞内镁的调节是一种生理学上的 重要的过程。 发展的最新进展 镁离子的荧光探针现在使得有可能 解决这个问题。 拟议的研究将利用 这些新的研究工具提供了基本的新信息， 关于镁的细胞内调节，最丰富的 哺乳动物细胞中发现的阳离子。