Molecular Genetic Analysis of Rp1-Mediated Disease Resistance in Maize

Rp 1的功能和结构特性将在 分子水平和互补研究。 个人 来自Rp 1区域的克隆将被精细结构定位， 到Rp 1功能组件，并筛选的位置 编码的抄本。 来源于潜在Rp 1的cDNA克隆 转录物将被分离并相对于基因组定位。 克隆 染色体步移将用于获得基因组克隆 可能完全编码Rp 1衍生的抗病性。 这些克隆将被转化成玉米，并进行测试。 能够赋予种族特异性抗性， 组织。*/

The functional and structural properties of Rp1 will be analyzed at the molecular level and by complementation studies. Individual clones from the Rp1 region will be fine-structure mapped relative to Rp1 functional components and screened for the location of encoded transcripts. cDNA clones derived from potential Rp1 transcripts will be isolated and mapped relative to the genomic clones. Chromosome walking will be used to acquire genomic clones that are likely to fully encode Rp1-derived disease resistance. These clones will be transformed into maize and tested for the ability to impart race-specific resistance to transformed tissues.***//