Molecular Genetic Analysis of Rp1-Mediated Disease Resistance in Maize

Rp1的功能和结构特性将在分子水平和通过互补研究进行分析。来自Rp1区域的单个克隆将相对于Rp1功能成分进行精细结构映射，并筛选编码转录本的位置。从潜在的Rp1转录本中获得的cDNA克隆将被分离并相对于基因组克隆进行定位。染色体行走将用于获得可能完全编码rp1衍生的抗病基因的基因组克隆。这些克隆将被转化为玉米，并测试其对转化组织赋予种族特异性抗性的能力

The functional and structural properties of Rp1 will be analyzed at the molecular level and by complementation studies. Individual clones from the Rp1 region will be fine-structure mapped relative to Rp1 functional components and screened for the location of encoded transcripts. cDNA clones derived from potential Rp1 transcripts will be isolated and mapped relative to the genomic clones. Chromosome walking will be used to acquire genomic clones that are likely to fully encode Rp1-derived disease resistance. These clones will be transformed into maize and tested for the ability to impart race-specific resistance to transformed tissues.***//