Acquisition of Equipment for a Comprehensive Agricultural Genomics Laboratory at Purdue University

请拨资金用于在普渡大学建立农业基因组学综合实验室所需的仪器设备。在过去的五年里，普渡大学加强了其在植物和微生物遗传学方面的能力。普渡大学遗传学项目是由大学资源和美国国家科学基金会植物遗传学培训基金创立并资助的。普渡大学现在计划通过建立一个全面的农业基因组学计划来扩大这一计划。目前用于基因组研究的仪器有大量的积压，迫切需要增加可用的资源，以适应随着农业基因组学计划的扩大而预期的需求增加。该奖项将购买的仪器分为三类：(1)DNA测序和分析以及基因组图谱；(2)信息学；(3)功能基因组学。基金将提供四台DNA测序仪及相关设备、两台SPARC计算机、两台微阵列机器以及额外的显微镜和辅助设备。该设备将支持对几种植物和真菌基因组的结构分析。对病原菌和植物寄主中表达的基因进行全面的cdna克隆将确定这些相互作用的主要成分。将采取类似的方法来研究次生产物合成、细胞壁发育、细胞骨架相互作用或蛋白质-DNA相互作用的分子遗传学。还将启动对标准土壤和污染土壤中微生物多样性的研究。

Funds are requested for instrumentation needed to establish a Comprehensive Agricultural Genomics Laboratory at Purdue University. Over the past five years, Purdue has strengthened its capabilities in plant and microbial genetics. The Purdue Genetics Program was founded and funded by University resources and an NSF Training Grant in Plant Genetics. Purdue now plans to expand this program with the establishment of a comprehensive program in agricultural genomics. Current instrumentation for genomic research runs with a substantial backlog and there is great need to enhance available resources to accommodate the expected increase in demand as the program in agricultural genomics expands. The instrumentation to be purchased by the award falls into three categories: (1) DNA sequence and analysis and genome mapping; (2) informatics; and (3) functional genomics. Funds will provide four DNA sequencers and associated equipment; two Sparc computers; two micro array machines and additional microscopes and support equipment. This equipment will support analysis of the structure of several plant and fungal genomes. Comprehensive cDNA cloning of genes expressed in both pathogens and their plant hosts will identify major components of these interactions. Similar approach will be taken to study the molecular genetics of secondary product synthesis, cell wall development, cytoskeletal interactions or protein-DNA interactions. Studies will also be initiated on microbial diversity in standard versus polluted soils.