Mechanisms of DNA Repair and Mutagenesis in Plants

在紫外光诱导的DNA损伤修复方面存在缺陷的拟南芥突变体将被分离。基因分析将用于阐明逆转或修复DNA损伤所需的各种途径的分子机制，并确定涉及哪些修复途径。重组缺陷的突变体也可以被分离出来，因为许多基本的细胞机制也利用修复途径。在突变体特征确定之后，将继续研究克隆相关基因的策略。这种模式生物的DNA修复突变体的可获得性将加强对植物这一重要研究领域的研究。

Mutants of Arabidopsis thaliana which are defective in the repair of DNA damage induced by ultraviolet light will be isolated. Genetic analysis will be used to elucidate the molecular mechanism of the various pathways required for reversal or repair of damage to DNA and determine which of the repair pathways are involved. Mutants defective in recombination can be expected to also be isolated since many basic cellular mechanisms also utilize the repair pathways. After mutant characterization, strategies for cloning the involved genes will be pursued. The availability of DNA repair mutants of this model organism will enhance studies of this important area of research in plants.***