Mechanisms of DNA Repair and Mutagenesis in Plants

植物 DNA 修复和诱变机制

• 批准号: 9019159
• 负责人: Anne Britt
• 金额: $ 15.48万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9019159&HistoricalAwards=false
• 关键词: Mechanisms; DNA; Repair; Mutagenesis; Plants

Mutants of Arabidopsis thaliana which are defective in the repair of DNA damage induced by ultraviolet light will be isolated. Genetic analysis will be used to elucidate the molecular mechanism of the various pathways required for reversal or repair of damage to DNA and determine which of the repair pathways are involved. Mutants defective in recombination can be expected to also be isolated since many basic cellular mechanisms also utilize the repair pathways. After mutant characterization, strategies for cloning the involved genes will be pursued. The availability of DNA repair mutants of this model organism will enhance studies of this important area of research in plants.***

拟南芥在紫外光诱导下DNA损伤修复缺陷的突变体将被分离。遗传分析将用于阐明逆转或修复DNA损伤所需的各种途径的分子机制，并确定涉及哪一种修复途径。重组缺陷突变体也有望被分离，因为许多基本的细胞机制也利用修复途径。突变体鉴定后，将寻求相关基因的克隆策略。这种模式生物的DNA修复突变体的可用性将加强这一重要研究领域在植物中的研究