权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Protein Structure and Folding at Low Temperature

蛋白质结构和低温折叠

基本信息

批准号：
9019530
负责人：
Anthony Fink
金额：
$ 26万
依托单位：
University of California-Santa Cruz
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1991
资助国家：
美国
起止时间：
1991-04-01 至 1995-03-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9019530&HistoricalAwards=false
关键词：
Protein Structure Folding Low Temperature

项目摘要

This proposal concerns two aspects of protein folding and stability: the nature of partially-folded species, especially the molten globule state, and the factors which determine a particular folding pathway. The long-term goals are to learn how the amino acid sequence of a polypeptide guides the folding to the native state, and determines the stability of protein conformations. A relatively compact protein conformational state, often referred to as the molten globule state, has been shown to be an intermediate in the refolding of certain proteins, and to be stable under certain denaturation conditions for some proteins. One of the goals of this research is to increase the understanding about the molecular details and physical properties of molten globules. The molten globule state will be studied under both equilibrium and transient refolding conditions. In addition the pathway of folding will be mapped out by using specific signals from different regions of the molecule in stopped-flow kinetics experiments at both ambient and subzero temperatures. A variety of biophysical techniques will be used to structurally characterize the molten globule state from several different proteins (in order to establish generalities); these include FTIR, circular dichroism (CD), fluorescence and hydrodynamic radius measurements. In addition kinetic experiments using stopped-flow fluorescence and CD will be performed.

该建议涉及蛋白质折叠的两个方面，稳定性：部分折叠物种的性质，特别是熔融球状态，以及决定一个特殊的折叠途径。长期目标是学习多肽的氨基酸序列如何引导折叠天然状态，并决定蛋白质的稳定性构象相对紧凑的蛋白质构象状态，通常被称为熔球状态，已经被显示为某些重折叠的中间体，蛋白质，并在一定的变性下保持稳定一些蛋白质的条件。其中一个目标是研究是为了增加对分子的理解，熔融小球的细节和物理性质。熔融小球状态将在平衡和瞬时重折叠条件。此外，折叠将通过使用来自停流动力学中分子的不同区域在环境温度和零度以下的温度下进行实验。一各种生物物理技术将被用于从结构上从几个不同的特征熔融球状态蛋白质（以建立一般性）;这些包括 FTIR、圆二色性（CD）、荧光和流体动力学半径测量此外，动力学实验使用将进行停流荧光和CD。