U.S.-Japan Cooperative Research: "Role of Molten Globule State in the Structure and Function of Proteins"

美日合作研究：“熔球态在蛋白质结构和功能中的作用”

• 批准号: 9016819
• 负责人: Anthony Fink
• 金额: $ 0.74万
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9016819&HistoricalAwards=false
• 关键词: U.S.; Japan; Cooperative; Research; Role

This award will facilitate continued collaboration between Professor Anthony L Fink, University of California, Santa Cruz, and Associate Professor Yugi Goto, Department of Biology, Osaka University, Japan. The scientists will investigate the protein conformational state known as the molten globule and its role in protein folding reactions. The goal of the research is to increase our understanding of the molecular details and physical properties of molten globules and to determine whether they may be important in certain physiological processes such as membrane transloc- ation. Research has indicated that molten globule-like states are of critical importance in protein folding, protein denaturation and probably in vivo protein function. In order to establish generalities, a variety of biophysical techniques will be used to structurally characterize the molten globule state from several different proteins. In addition, kinetic experiments using stopped-flow fluorescence and CD will be performed. An increase in our understanding of the deter- minants of protein folding and stability has important implications for most aspects of proteins and their functions, including drug design and proteins for medical and industrial uses. The US and Japanese scientists involved in this effort have complementary skills and experience in studying structural and functional properties of proteins and enzymes. Both have obtained significant funds for their individual efforts on this problem and their previous collaborations have been highly productive, and their research original and important. Their continued joint efforts should contribute to our understanding of proteins in a wide variety of ways.

该奖项将促进双方之间的持续合作。 教授安东尼L芬克，加州大学，圣诞老人 克鲁兹和副教授Yugi后藤，部门 日本大坂大学生物学。 科学家将 研究蛋白质的构象状态， 熔融球及其在蛋白质折叠反应中的作用。 这项研究的目的是增加我们对 熔体的分子结构和物理性质 并确定它们是否可能在 某些生理过程，如膜transloc， 行动。 研究表明， 状态在蛋白质折叠中至关重要，蛋白质 变性和可能的体内蛋白质功能。为了 为了建立普遍性，各种生物物理技术 将被用来从结构上表征熔融球 几种不同的蛋白质。 此外，Kinetic 使用停流荧光和CD的实验将是 执行。 在我们的理解中， 蛋白质折叠和稳定性的重要因素 对蛋白质及其功能的大多数方面的影响， 包括药物设计和用于医疗和工业的蛋白质 使用. 参与这项工作的美国和日本科学家 学习结构的互补技能和经验 以及蛋白质和酶的功能特性。 都有 为他们的个人努力获得了大量资金， 这个问题和他们之前的合作 高生产力，他们的研究原创和重要。 他们的持续共同努力应有助于我们的 以多种方式了解蛋白质。