SCINTILLATION PROXIMITY FLUOROGRAPHY: A NEW METHOD FOR TRACKING BIOMOLECULES IN CELLS

该奖项的目的是测试使用与生物素化抗体、放射性大分子前体和常规电子显微镜放射自显影技术相结合的闪光剂来检测新合成的大分子的可行性。这种方法如果被开发出来，将允许研究人员跟踪特定分子的动态，从它们的合成地点到细胞内的目的地。可行性试验包括：(1)Rubisco在浮萍叶绿体中的定位；(2)过氧化氢酶在过氧化体中的定位；(3)液泡ATPase在植物根尖中的定位和跟踪。

The objective of this award is to test the feasibility of the use of scintillant coupled to biotinylated antibodies, radioactive macromolecular precursors and conventional electron microscope autoradiography for the detection of newly-synthesized macromolecules. The method, if developed, will allow researchers to follow the dynamics of specific molecules from their site of synthesis to their destination within the cell. The feasibility test will be carried out on: (1) localization of rubisco in chloroplasts of Lemna, (2) localization of catalase in peroxisomes, and (3) localization and tracking of vacuolar ATPase in plant root tips.