Ultra Low Temparature Electron Microscopy and Spectroscopy

超低温电子显微镜和光谱学

• 批准号: 9019709
• 负责人: Kenneth Taylor
• 金额: $ 9万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9019709&HistoricalAwards=false
• 关键词: Ultra; Low; Temparature; Electron; Microscopy

We will study the stability of specific chemical elements and measure the mass of certain types of biological substances and embedding materials as a function of irradiation and temperature. We will use an electron energy loss spectrometer interfaced to a computer to make quantitative measurements. While this work is going on, we will develop a more efficient data acquisition system based on new interfaces and computer programs. To measure very cold specimens, we will mount our spectrometer on a special electron microscope with a superconducting objective lens operating at liquid helium temperature. This will help future research efforts because very low temperature will probably stabilize some elements, hence improving the accuracy of concentration measurements and allowing the use of certain chemical elements as site-specific stains. We will learn which elements are "caged at low temperature or "frozen" onto surfaces. Improved stability of embedding materials will allow more accurate three-dimensional views of sectioned specimens. Studies of major classes of molecules (like DNA) will indicate which materials are most helped by low temperature. Development of new instrumentation will improve the efficiency of our studies of radiation effects and will allow our techniques to be used more widely by other.

我们将研究特定化学元素的稳定性，并测量某些类型的生物物质和包埋材料的质量随辐照和温度的变化。我们将使用与计算机连接的电子能量损失谱仪进行定量测量。在这项工作进行的同时，我们将开发一个基于新接口和计算机程序的更有效的数据采集系统。为了测量非常冷的样品，我们将把光谱仪安装在一个特殊的电子显微镜上，显微镜上有一个在液氦温度下工作的超导物镜。这将有助于未来的研究工作，因为极低的温度可能会稳定一些元素，从而提高浓度测量的准确性，并允许使用某些化学元素作为特定地点的污渍。我们将学习哪些元素被“关在低温笼子里”或“冷冻”在表面上。嵌入材料稳定性的提高将使切片标本的三维视图更加精确。对主要分子类别（如DNA）的研究将表明低温对哪些材料最有帮助。新仪器的开发将提高我们研究辐射效应的效率，并将使我们的技术得到更广泛的应用。