Regulation of Nitrate Reductase Activity and Protein Levels in Higher Plants

高等植物硝酸还原酶活性和蛋白质水平的调节

• 批准号: 9020773
• 负责人: Wilbur Campbell
• 金额: $ 5万
• 依托单位: Michigan Technological University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1992-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9020773&HistoricalAwards=false
• 关键词: Regulation; Nitrate; Reductase; Activity; Protein

The research planned is directed toward increasing understanding of regulation of nitrate reductase activity in higher plants. The specific objective of this project is to analyze, at the molecular level, the flavin domain of corn leaf nitrate reductase. The recombinant form of this enzyme is produced in a bacterium. An aspect of this analysis is to generate a vector with an appropriate gene insert so that both the flavin and cytochrome domains of the enzyme will be expressed in the bacterium, thus making possible easy production of large amounts of the enzyme. Plants assimilate the nitrate-nitrogen of soil and water into their cell materials initially be means of the enzyme nitrate-reductase. The details of the regulation of the formation of this enzyme are incomplete, and the aim of this project is to enlarge our understanding of this significant process.

计划中的研究旨在增进对 硝酸还原酶活性的调节。 该项目的具体目标是分析，在 分子水平，玉米叶片硝酸还原酶的黄素结构域。 这种酶的重组形式在细菌中产生。 该分析的一个方面是生成具有 适当的基因插入，使黄素和细胞色素 酶的结构域将在细菌中表达，因此 使得可以容易地生产大量的酶。 植物吸收土壤和水中的硝态氮， 细胞材料最初是指硝酸还原酶。 该酶形成的调节细节如下： 不完整，这个项目的目的是扩大我们的 了解这个重要的过程。