Higher Plant Nitrate Reductase: Structure, Mechanism and Modification

高等植物硝酸还原酶：结构、机制和修饰

• 批准号: 8803998
• 负责人: Wilbur Campbell
• 金额: $ 12.4万
• 依托单位: Michigan Technological University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8803998&HistoricalAwards=false
• 关键词: Higher; Plant; Nitrate; Reductase; Structure

Nitrate reductase (NR) is the enzyme that reduces soil nitrate to nitrite, a key step in the utilization of inorganic nitrogen fertilizer by plants. This proposal is aimed at elucidating the structure and mechanism of action of NR, which has been purified to homogeneity using affinity chromatography and monoclonal antibody columns. The NR gene from squash has been cloned. Efforts will be made to express this gene in a suitable host so that NR can be obtained in quantity for crystallization studies. The enzyme will be subjected to limited proteolysis and the fragments sequenced in order to identify the active sites. The results will enhance our understanding of how NR works, and may eventually enable plants to utilize nitrogen fertilizer more efficiently.

硝酸还原酶（NR）是还原土壤中硝酸盐的酶。 硝酸盐到亚硝酸盐，在利用的关键步骤 无机氮肥料。 这项建议是 旨在阐明的结构和作用机制， NR，其已使用亲和层析纯化至均一 层析和单克隆抗体柱。 NR基因 已经被克隆了 将努力表达 将该基因导入合适的宿主中，从而可以在 用于结晶研究的量。 这种酶将 进行有限的蛋白水解，并对片段进行测序 以识别活性位点。 这些结果将增强我们对NR如何工作的理解， 并最终使植物能够利用氮 肥料更有效。