Integrated Undergraduate Laboratories for Biochemistry and Molecular Biology

生物化学和分子生物学综合本科实验室

• 批准号: 9152722
• 负责人: Wilbur Campbell
• 金额: $ 5万
• 依托单位: Michigan Technological University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1993-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9152722&HistoricalAwards=false
• 关键词: Integrated; Undergraduate; Laboratories; Biochemistry; Molecular

A key challenge in educating today's undergraduate students is to bring together understanding of biochemistry, immunochemistry, microbiology and molecular biology in a functional manner. The aim of this project is to develop a year-long laboratory course with 3 integrated modules for undergraduate majors in the Department of Biological Sciences' Bachelor of Science degree programs in Biochemistry and Microbiology which are now being expanded to include Molecular Biology. These laboratory modules are designed to provide up- to-date training for the students in fundamental methods used in biochemistry, immunochemistry, microbiology an molecular biology. This laboratory course sequence will complement the year-long lecture course in Biochemistry and Molecular Biology, which is a set of 3 one-term modules required for students studying Biochemistry and Microbiology. The current situation is that only a single one-term laboratory course is offered and it covers only the basic methods used in biochemistry and a brief introduction to nucleic acids. The proposed project is a logical extension of our curriculum, which would bring together contemporary methodology using modern instrumentation to enhance the learning environment for these students. To achieve this program development, we will integrate our offerings in laboratory methods to link the student's understanding of biochemistry, immunochemistry and microbiology to molecular biology. The major instruments to be purchased include: UV-Vis spectrophotometers, PhastSystem gel electrophoresis system, FPLC chromatography system (including an IBM-type personal computer), preparative centrifuge, bench-top fermenter, DNA sequencing gel apparatus and DNA sequence analysis software. The significance of this project lies in the unique integration around a central theme of biochemical and immunochemical methods for analysis of enzymes and recombinant gene products, the regulation of gene expression in bacteria, and the cloning and characterization of bacterial genes.

教育当今本科生的一个关键挑战是 将生物化学的理解结合起来， 免疫化学、微生物学和分子生物学 功能性的方式。该项目的目的是开发一个 为期一年的实验室课程，包括3个综合模块， 生物科学系本科专业 理学学士学位课程在生物化学和 微生物学，现在正在扩大到包括分子 生物学这些实验室模块旨在提供- 对学生进行基本方法的最新培训 在生物化学、免疫化学、微生物学和分子生物学中， 生物学本实验室课程序列将补充 为期一年的生物化学和分子生物学讲座课程， 这是一套3个一学期的模块，要求学生 学习生物化学和微生物学。现状 只有一个学期的实验室课程， 它只包括生物化学中使用的基本方法， 核酸简介拟议的项目是一个 我们课程的逻辑延伸，这将使我们的课程 使用现代仪器的当代方法， 改善这些学生的学习环境。实现 在这个项目的开发中，我们将把我们的产品整合到 实验室的方法来连接学生的理解， 免疫化学和微生物学 生物学 采购的主要仪器包括： 电泳仪，PhastSystem凝胶电泳系统， FPLC色谱系统（包括IBM型个人 计算机），制备离心机，台式发酵罐，DNA 测序凝胶仪和DNA序列分析软件。 这个项目的意义在于独特的融合 围绕着生物化学和免疫化学的中心主题 酶和重组基因产物的分析方法， 细菌中基因表达的调控，以及克隆 和细菌基因的表征。