Integrated Undergraduate Laboratories for Biochemistry and Molecular Biology

当今教育本科学生的一个关键挑战是以功能性的方式将生物化学、免疫化学、微生物学和分子生物学的理解结合起来。该项目的目的是为生物科学系的生物化学和微生物学理学学士学位课程的本科专业开发一门为期一年的实验课程，其中包括三个综合模块，目前已扩展到分子生物学。这些实验模块旨在为学生提供最新的生物化学、免疫化学、微生物学和分子生物学的基本方法培训。本实验课将补充为期一年的生物化学和分子生物学讲座课程，该课程是学生学习生物化学和微生物学所需的一套三个学期的模块。目前的情况是，只开设一学期的实验课程，只涵盖生物化学的基本方法和核酸的简单介绍。拟议的项目是我们课程的逻辑延伸，它将结合现代方法和现代仪器，为这些学生提供更好的学习环境。为了实现这一计划的发展，我们将整合我们的实验室方法，将学生对生物化学，免疫化学和微生物学的理解与分子生物学联系起来。主要采购仪器包括：紫外可见分光光度计、PhastSystem凝胶电泳系统、FPLC色谱系统（含一台ibm型个人电脑）、制备离心机、台式发酵罐、DNA测序凝胶仪、DNA序列分析软件。该项目的意义在于围绕生化和免疫化学方法的中心主题进行独特的整合，以分析酶和重组基因产物，调节细菌基因表达，以及克隆和表征细菌基因。

A key challenge in educating today's undergraduate students is to bring together understanding of biochemistry, immunochemistry, microbiology and molecular biology in a functional manner. The aim of this project is to develop a year-long laboratory course with 3 integrated modules for undergraduate majors in the Department of Biological Sciences' Bachelor of Science degree programs in Biochemistry and Microbiology which are now being expanded to include Molecular Biology. These laboratory modules are designed to provide up- to-date training for the students in fundamental methods used in biochemistry, immunochemistry, microbiology an molecular biology. This laboratory course sequence will complement the year-long lecture course in Biochemistry and Molecular Biology, which is a set of 3 one-term modules required for students studying Biochemistry and Microbiology. The current situation is that only a single one-term laboratory course is offered and it covers only the basic methods used in biochemistry and a brief introduction to nucleic acids. The proposed project is a logical extension of our curriculum, which would bring together contemporary methodology using modern instrumentation to enhance the learning environment for these students. To achieve this program development, we will integrate our offerings in laboratory methods to link the student's understanding of biochemistry, immunochemistry and microbiology to molecular biology. The major instruments to be purchased include: UV-Vis spectrophotometers, PhastSystem gel electrophoresis system, FPLC chromatography system (including an IBM-type personal computer), preparative centrifuge, bench-top fermenter, DNA sequencing gel apparatus and DNA sequence analysis software. The significance of this project lies in the unique integration around a central theme of biochemical and immunochemical methods for analysis of enzymes and recombinant gene products, the regulation of gene expression in bacteria, and the cloning and characterization of bacterial genes.