Factors Influencing the Stability of Catabolic Plasmids

影响分解代谢质粒稳定性的因素

• 批准号: 9020944
• 负责人: C. Grady
• 金额: $ 27.25万
• 依托单位: Clemson University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-15 至 1993-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9020944&HistoricalAwards=false
• 关键词: Factors; Influencing; Stability; Catabolic; Plasmids

This is an award to provide support for a project to determine conditions under which the performance of a wastewater biological treatment process is adversely affected by loss of plasmids that are encoded for wastewater constituents during their temporary absence from the wastewater stream. The investigator plans to conduct both batch and continuous culture experiments using tolualdehyde (degraded by plasmid-encoded enzymes), benzoate (degraded by plasmid- or chromosomally-encoded enzymes), and lysine (degraded by chromosomally-encoded enzymes), singly and in pairs to determine how the nature of the substrate and the specific growth rate of the culture influence the rate constants that describe plasmid loss. Knowledge of factors that control the rate at which plasmids are lost will permit the engineering design of treatment systems to minimize their loss and otherwise compensate for it. This knowledge will also beneficially affect the operating efficiency of existing wastewater treatment plants that depend on biological processes for their operation.

这是一个奖项，为一个项目提供支持， 确定履行义务的条件 废水生物处理工艺 受到质粒丢失的影响， 暂时不存在的废水成分 来自废水流。 研究者计划 进行分批和连续培养实验 使用甲苯甲醛（被质粒编码的酶降解）， 苯甲酸酯（由质粒或染色体编码的 酶）和赖氨酸（由染色体编码的 酶），单独和成对，以确定如何性质 和比生长速率的影响。 培养影响描述质粒的速率常数 损失 了解控制死亡率的因素 质粒丢失将允许工程设计， 处理系统，以尽量减少其损失， 补偿它。这些知识也将有益于 影响现有废水的运行效率 依赖生物过程的处理厂 他们的行动。