Fluorescence in situ PCR (FLIP) in Individual Cells of Marine Microalgae

该项目代表了“海洋生物技术和海洋科学研究奖学金”。研究员Yentsch博士将接受实验室课程形式的正式培训，然后在缅因州巴尔港的杰克逊实验室接受学徒培训，由赞助的资深科学家查尔斯·西德曼博士指导。培训包括学习分子生物学的实验室技术，特别是这些技术将包括使用限制酶、印迹、克隆、测序和聚合酶链式反应(PCR)。该项目的科学重点是学习和改进/开发技术，以评估单细胞微藻中基因表达的异质性和突变率。最终，在额外资金的支持下，C.M.Yentsch博士和教员赞助人C.Sidman博士希望将透析培养放在人类受到不同程度侮辱的环境中，并通过使用流式细胞仪的荧光原位聚合酶链式反应(FliP)来检测单细胞中基因表达的异质性。

This project represents a "Research Fellowship in Marine Biotechnology and the Ocean Sciences. Dr. Yentsch, the research fellow, will receive formal training in a laboratory course format followed by an apprenticeship at, The Jackson Laboratory, in Bar Harbor, Maine under the supervision of a sponsoring senior scientist, Dr. Charles Sidman. The training encompasses learning laboratory techniques in molecular biology, specifically these techniques will include use of restriction enzymes, blotting, cloning, sequencing and polymerase chain reaction (PCR). The scientific focus of the project is to learn and refine/develop techniques in order to assess heterogeneity of gene expression and mutation rate in single celled microalgae. Eventually with additional funding, the fellow Dr. C.M. Yentsch and faculty sponsor Dr. C. Sidman expect to place dialysis cultures in environments with varying degrees of human insult and assay for heterogeneity of gene expression in single cells via fluorescent in situ PCR (FLIP) using flow cytometry.