Fluorescence in situ PCR (FLIP) in Individual Cells of Marine Microalgae

海洋微藻单个细胞的荧光原位 PCR (FLIP)

• 批准号: 9023838
• 负责人: Clarice Yentsch
• 金额: $ 5.37万
• 依托单位: Bigelow Laboratory for Ocean Sciences
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9023838&HistoricalAwards=false
• 关键词: Fluorescence; situ; PCR; FLIP; Individual

This project represents a "Research Fellowship in Marine Biotechnology and the Ocean Sciences. Dr. Yentsch, the research fellow, will receive formal training in a laboratory course format followed by an apprenticeship at, The Jackson Laboratory, in Bar Harbor, Maine under the supervision of a sponsoring senior scientist, Dr. Charles Sidman. The training encompasses learning laboratory techniques in molecular biology, specifically these techniques will include use of restriction enzymes, blotting, cloning, sequencing and polymerase chain reaction (PCR). The scientific focus of the project is to learn and refine/develop techniques in order to assess heterogeneity of gene expression and mutation rate in single celled microalgae. Eventually with additional funding, the fellow Dr. C.M. Yentsch and faculty sponsor Dr. C. Sidman expect to place dialysis cultures in environments with varying degrees of human insult and assay for heterogeneity of gene expression in single cells via fluorescent in situ PCR (FLIP) using flow cytometry.

该项目为“海洋生物技术和海洋科学研究奖学金”。研究员Yentsch博士将以实验室课程的形式接受正式培训，然后在缅因州巴尔港的杰克逊实验室（the Jackson laboratory）接受资深科学家Charles Sidman博士的指导。培训内容包括学习分子生物学的实验室技术，特别是这些技术将包括限制性内切酶的使用、印迹、克隆、测序和聚合酶链反应（PCR）。该项目的科学重点是学习和改进/开发技术，以评估单细胞微藻基因表达的异质性和突变率。最终，在获得额外资金的情况下，研究员C.M. Yentsch博士和教师赞助人C. Sidman博士希望将透析培养物置于不同程度的人类侮辱环境中，并利用流式细胞术通过荧光原位PCR （FLIP）检测单细胞中基因表达的异质性。