Fluorescence in situ PCR (FLIP) in Individual Cells of Marine Microalgae

该项目是一项“海洋研究奖学金”， 生物技术和海洋科学。杨奇博士， 研究员，将在实验室课程中接受正式培训 在杰克逊实验室实习， 在缅因州的巴尔港，在一位赞助者的监督下， 科学家查尔斯·西德曼博士 培训包括学习 分子生物学的实验室技术，特别是这些 技术将包括使用限制酶，印迹， 克隆、测序和聚合酶链反应（PCR）。 该项目的科学重点是学习和 改进/开发技术，以评估 基因表达和突变率。 最终，随着资金的增加，C.M.博士。延奇 和教师赞助商C博士西德曼希望将透析 文化在不同程度的人类侮辱的环境中， 单细胞中基因表达异质性的测定 使用流式细胞术的荧光原位PCR（FLIP）。

This project represents a "Research Fellowship in Marine Biotechnology and the Ocean Sciences. Dr. Yentsch, the research fellow, will receive formal training in a laboratory course format followed by an apprenticeship at, The Jackson Laboratory, in Bar Harbor, Maine under the supervision of a sponsoring senior scientist, Dr. Charles Sidman. The training encompasses learning laboratory techniques in molecular biology, specifically these techniques will include use of restriction enzymes, blotting, cloning, sequencing and polymerase chain reaction (PCR). The scientific focus of the project is to learn and refine/develop techniques in order to assess heterogeneity of gene expression and mutation rate in single celled microalgae. Eventually with additional funding, the fellow Dr. C.M. Yentsch and faculty sponsor Dr. C. Sidman expect to place dialysis cultures in environments with varying degrees of human insult and assay for heterogeneity of gene expression in single cells via fluorescent in situ PCR (FLIP) using flow cytometry.