Development of a Flow Cytometry/Cell Sorting Facility

流式细胞术/细胞分选设备的开发

• 批准号: 8800855
• 负责人: Clarice Yentsch
• 金额: $ 22.21万
• 依托单位: Bigelow Laboratory for Ocean Sciences
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8800855&HistoricalAwards=false
• 关键词: Development; Flow; Cytometry; Cell; Sorting

The Flow Cytometry and Cell Sorting Facility at the Bigelow Laboratory of Ocean Sciences will further develop the operation and methodology of flow cytometric applications to marine biology/oceanography. This multi-user facility has been instrumental in adapting flow cytometry, a technology developed for use in the biomedical research community, for ocean science research. With this technique, a stream of phytoplankton cells or other microscopic particles is passed through a laser light beam. Different cells and particles can be enumerated and classified according to how they fluoresce or react in response to the laser light. Analysis of the fluoresced signal can yield important information on cell size, cellular constituents, and metabolic condition. Work will continue to develop appropriate flow cytometry standards and items useful for intercalibration of phyto- plankton data. The Facility will be available for use by guest investigators. Coordination with industry will promote further development and modifications to basic flow cytometry instrumentation for oceanographic research. Improvements and modifications will be directed towards towards a) expanding dynamic range of the instruments (4-decade log representa- tion), b) increasing the specific volume analyzed, c) incorporating volume sensing as well as light scatter, d) automating an on-line sampler for a pump profiling system, and e) making the system more rugged for shipboard use.

毕格罗海洋科学实验室的流式细胞术和细胞分选设施将进一步开发流式细胞术在海洋生物学/海洋学中的应用的操作和方法。 这个多用户设施在流式细胞术的应用方面发挥了重要作用，流式细胞术是一种为生物医学研究界和海洋科学研究而开发的技术。 通过这项技术，浮游植物细胞或其他微观颗粒流穿过激光束。 不同的细胞和颗粒可以根据它们对激光的响应方式发出荧光或发生反应来进行计数和分类。 对荧光信号的分析可以产生有关细胞大小、细胞成分和代谢状况的重要信息。 将继续开发适当的流式细胞术标准品和可用于浮游植物数据相互校准的项目。 该设施可供客座调查员使用。 与工业界的协调将促进海洋学研究的基本流式细胞仪仪器的进一步开发和修改。 改进和修改将针对 a) 扩大仪器的动态范围（4 个十进制对数表示），b) 增加分析的比容，c) 结合体积传感和光散射，d) 自动化泵分析系统的在线采样器，以及 e) 使系统更加坚固耐用，适合船上使用。