Science in Developing Countries Program: The Systematics andEvolution of the Genus Coreopsis (Asteraceae) in South America

该项目将支持俄亥俄州立大学的丹尼尔·J·克劳福德博士在系统生物学方面的合作研究，阿肯色大学的Edwin B.Smith博士和秘鲁特鲁希略大学男高音Orrego de Trujillo大学的Aundio Sagastegui博士。该项目将启动对菊属(菊科)南美代表的系统学和进化的研究。来自南美洲的34个物种被描述，其中绝大多数发生在秘鲁的北部安第斯山脉，所有物种都被归入该教派。假木耳。除了阿尔法水平之外，人们对该种知之甚少，大多数分类群主要是根据叶的形态描述的。合作工作将涉及以下方法：评估所描述物种内部和之间的形态变异的实地研究，记录这种变异的标本收集，采集花蕾，采集种子和干燥的叶材料；确定每个物种的染色体数目；使用酶电泳法估计物种内的遗传变异和物种间的差异；并利用形态和叶绿体DNA限制性内切酶位点变异构建了南美金鸡儿属和所有金鸡儿属的系统发育假说。该项目将提供对金鸡儿属系统进化方面的洞察，并将允许三名资质良好的调查人员之间发展协同关系。该项目将提供秘鲁首席调查员目前无法获得的资源和研究机会，并将有秘鲁学生参与。由于这类研究还提供了知识的快速交流和传播，该项目将实现发展中国家科学计划的目标。

This project will support collaborative studies in systematic biology by Dr. Daniel J. Crawford of Ohio State University, Dr. Edwin B. Smith of the University of Arkansas and Dr. Aundio Sagastegui of the Universidad Antenor Orrego de Trujillo, Peru. The project will initiate studies of the systematics and evolution of South American representatives of the Genus Coreopsis (Asteraceae). Thirty four species are described from South America with the vast majority occurring in the northern Andes of Peru and all assigned to the sect. Pseudoagarista. Little is known about the species beyond the alpha-level, with most of the taxa described primarily on leaf morphology. The collaborative effort will involve the following methods: field studies to assess morphological variation within and among the described species, specimen collection to document this variation, gathering of floral buds and collecting seeds and dried leaf material; determination of chromosome numbers for each species; the use of enzyme electrophoresis to estimate genetic variation within and divergence among species; and the use of morphology and chloroplast DNA restriction site variation to construct phylogenic hypotheses for South American Coreopsis and for all of Coreopsis. The project will provide insight into systematic-evolutionary aspects of the genus Coreopsis and will allow a synergistic relationship to develop among the three well- qualified investigators. The project will present resources and research opportunities currently unavailable to the Peruvian principal investigator and will involve Peruvian students. Since this type of research also provides for the rapid exchange and dissemination of knowledge, the project will fulfill the goals of the Science in Developing Countries Program.