Enzyme Electrodes Based on Photoactivated Conducting Organic Polymers

导电有机聚合物是一类新型的低成本轻质导电材料，具有大量重要的实际应用。这些聚合物是制造具有独特功能的酶基电化学生物传感器的一种特别感兴趣的手段。然而，目前用于在电极表面形成这些聚合物薄膜的电化学方法的几个方面对它们在酶电极中的应用施加了严重的限制。一种形成导电聚合物（如聚吡咯）的全新方法已经被开发出来。该方法非常适合于酶电极的制备。将氧化还原酶的反应转化为电信号的新方法将从这种方法中产生。将使用黄嘌呤氧化酶和l -氨基酸氧化酶进行可行性研究，以确定它是否可以固定到通过非电过程形成的导电聚合物薄膜中。这些酶对其底物的活性将被检查。这种新方法将为利用柔性光沉积技术形成酶电极奠定基础，并可用于大规模生产导电聚合物。

Electrically conducting organic polymers are a new class of low-cost lightweight conducting materials that have a large number of important practical applications. These polymers are of particular interest as a means of fabricating enzyme-based electrochemical biosensors that have unique capabilities. However, several aspects of the electrochemical methods presently used for the formation of these polymers as thin films at electrode surfaces impose severe restrictions for their use in enzyme electrodes. A radically new method for forming conducting polymers, such as polypyrrole, has been developed. This method is highly suited to the fabrication of enzyme electrodes. New methods for transducing the reaction of an oxidoreductase enzyme into an electrical signal will arise from this approach. A feasibility study will be performed using xanthine oxidase and L-amino acid oxidase to determine if it can be immobilized into conducting polymer films formed by the non-electrical process. The activity of the enzymes toward their substrates will be examined. This new method will form the basis for the formation of enzyme electrodes using a flexible photodeposition technique, and can be used for the production of conducting polymers on a large scale.