Long and Medium-Term Research: Chloroplast Enslavement in Marine Oligotrich Ciliates

长期和中期研究：海洋寡毛虫纤毛虫的叶绿体奴役该奖项是根据长期和中期研究计划颁发的。外国卓越中心的中期研究，使美国科学家和工程师能够在国外公认的卓越研究中心进行为期三到十二个月的研究。该项目的奖项提供了联合研究的机会，并提供了使用国外独特或互补的设施、专业知识和实验条件的机会。该奖项将支持John Dolan博士与法国滨海维尔弗利奇动物园研究部主任Fereidoun Rassoulzadean进行为期12个月的博士后研究访问。研究人员将专注于寡毛类纤毛虫，它们经常主导海洋微型浮游生物群落，最近的研究表明，关于这些浮游生物的比例很大(。1/3)是混合营养的；也就是说，除了食草性外，它们还利用从摄入的藻类中隔离出来的叶绿体产生的光合作用。研究人员建议通过野外工作和实验室工作来询问是什么大规模环境特性影响了这些形式的丰度，以及什么细胞机制可能参与了叶绿体奴役。实地工作将检验混合营养寡毛藻的变异性与自养纳米浮游生物、光照和异养竞争对手丰度变化的关系。实验室工作将研究细胞机制。一种假设是，从叶绿体的藻类来源获得的化学线索改变了食物液泡处理的正常过程，这是合理的。概述了一系列旨在定位化学线索并检查来源藻类状态以及寡毛藻状态的影响的实验。这项研究将大大增加一些浮游生物如何在营养稀薄的环境中生存的知识，并将进一步了解与共生有关的细胞机制。奖励建议提供经费，用于支付国际旅费和家属津贴。

Long & Medium-Term Research: Chloroplast Enslavement in Marine Oligotrich Ciliates This award is under the Program for Long-& Medium Term Research at Foreign Centers of Excellence, which enables U.S. scientists and engineers to conduct three to twelve months of research abroad at research centers of proven excellence. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit by Dr. John Dolan with Fereidoun Rassoulzadegan, Director of Research at the Station Zoologique, Villefranche- sur-Mer, France. The researchers will focus on the oligotrich ciliates which often dominate marine microzo>plankton communities, about which recent work has shown a large fraction (. 1/3) are mixotrophic; that is, in addition to herbivory they use photosynthate produced by chloroplasts sequestered from ingested algae. The researchers propose to use field work and laboratory work to ask what large-scale environmental properties influence the abundance of these forms and what cellular mechanisms may be involved in chloroplast enslavement. Field work will examine the variability of mixotrophic oligotrichs in relation to changes in the abundance of autotrophic nanoplankton, light, and heterotrophic competitors. Laboratory work will investigate cellular mechanisms. The postulate that a chemical cue, derived from the algae source of chloroplasts, modifies the normal course of food vacuole processing is justified. A set of experiments designed to locate the chemical cue and examine the effect of the state of the source algae as well as the state of the oligotrich is outlined. The study will add significantly to knowledge of how some plankters survive in nutritionally dilute environ- ments and also will further understanding of the cellular mechanisms involved with symbiosis. The award recommendation provides funds to cover international travel and a dependents' allowance.