Analysis of Genes That Contribute to Embryogenesis in the Zebrafish

斑马鱼胚胎发生基因分析

• 批准号: 9105585
• 负责人: David Grunwald
• 金额: $ 33万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105585&HistoricalAwards=false
• 关键词: Analysis; Genes; Contribute; Embryogenesis; Zebrafish

The goals of the proposed study are 1) to identify genes that contribute to cell differentiation and formation of the body plan during embryogenesis of the zebrafish, Brachydanio rerio, and 2) to characterize when and where in the embryo cell or region specific gene expression originates. To examine gene function during development, Dr. Grunwald will generate transgenic zebrafish that harbor promoter-less lacZ reporter genes integrated at random locations in the genome. Expression of the reporter gene will necessarily be derived from endogenous transcriptional promoters. Patterns of gene expression that signal early embryonic differentiation events will be recognized by virtue of the expression of the lacZ transgene. Developmental mutation that arise from disruption of host genes and that disturb the normal structure of the one day embryo will be recognized using simple anatomical and behavioral screens. The developmental mutations and their associated phenotypes will be characterized in detail. Mutations of interest will be molecularly isolated and analyzed. To characterize in detail the ontogeny of the earliest tissues that arise during zebrafish embryogenesis, Dr. Grunwald will examine the expression of well defined tissue specific genes. Analysis of the expression of Vg1 will contribute to an understanding of the maternal contribution to development in animals with pluripotent blastomeres. Establishing when and where tissue specific gene expression originates will help clarify the relation between cell movements at gastrulation and the induction of tissue differentiation, and will be a critical first step toward developing experimentally testable hypotheses regarding the forces that promote tissue differentiation in the zebrafish embryo.

这项研究的目标是：1）鉴定出 有助于细胞分化和身体计划的形成 在斑马鱼（Brachydanio rerio）的胚胎发生期间，以及2） 以表征胚胎细胞或区域中的时间和位置 特异性基因表达开始。为了检测基因功能 在开发过程中，格伦沃尔德博士将产生转基因 携带无启动子lacZ报告基因的斑马鱼 整合在基因组的随机位置。表达 报告基因必然来源于内源性 转录启动子基因表达的模式， 将识别早期胚胎分化事件信号 由于lacZ转基因的表达。发育 由宿主基因破坏引起的突变， 扰乱一天胚胎的正常结构， 通过简单的解剖学和行为学的屏幕来识别。的 发育突变及其相关的表型将是 详细描述。感兴趣的突变将是 分子分离和分析。为了详细描述 斑马鱼早期组织的个体发育 在胚胎发生过程中，Grunwald博士将检查 组织特异性基因。Vg1的表达分析 将有助于理解母亲的贡献 多能卵裂球动物的发育。 确定组织特异性基因表达的时间和地点 起源将有助于阐明细胞运动之间的关系 在原肠胚形成和组织分化的诱导，和 将是实验性开发的关键第一步 关于促进组织生长的力的可检验假设 斑马鱼胚胎的分化。