Molecular Basis of Growth in Plants: Probing the Role of Wall Polysaccharides and Their Metabolism by Wall Proteins

植物生长的分子基础：探讨壁多糖的作用及其壁蛋白的代谢

• 批准号: 9106136
• 负责人: Donald Nevins
• 金额: $ 12.17万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-15 至 1994-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9106136&HistoricalAwards=false
• 关键词: Molecular; Basis; Growth; Plants; Probing

Plant cell elongation is an expression of metabolic changes that alter load bearing bonds within the wall to allow molecular displacement under the influence of internal pressures. Specifically, the proposal addresses the hypothesis that specific wall polysaccharide structures are modified in such a manner that such load bearing bonds are affected. The assay system utilized will be the Zea mays coleoptile. The major directions the proposal explores include: 1) The development of an in vitro system to quantitatively evaluate polysaccharide binding to cellulose. This model will allow a determination of the extent to which binding is affected by pH and the effect of pH after modification of structures by enzymes. 2) The experiments will extend the observation that antibodies are powerful probes to explore cell wall metabolism. The results derived from application of a family of glucan and glucanase antibodies will be extended to determine the consequences of exposure of tissue to glucuronoarabinoxylan antibodies. The behavior of ferulic acid and the role of diferulic acid linked to these molecules will be assessed. 3) An important component of (2) will be the application of a new enzyme mechanism, using glucuronoxylanase (previously called feraxanase), to modify glucuronoarabinoxylans to determine structure (especially glucuronic acid and ferulic acid components). A second emphasis is based on the observation that spatial (directional) application of antibodies is important in their capacity to suppress growth. This effort will help to determine the role of the epidermis in controlling elongation. Structural and metabolic aspects will also be examined in isolated epidermal cell walls. The experiments described in this proposal will help to elucidate the molecular mechanism of plant growth.//

植物细胞伸长是代谢变化的一种表现， 改变壁内的承载键， 在内部压力影响下的位移。 具体而言，该提案涉及的假设是， 壁多糖结构以这样的方式被修饰， 这种承载结合受到影响。 使用的测定系统 就是玉米胚芽鞘 建议的主要方向 探索包括：1）体外系统的开发， 定量评价多糖与纤维素的结合。 这 模型将允许确定约束力的程度， pH值的影响以及改性后pH值的影响 酶的结构。 2)这些实验将扩大 观察到抗体是探索细胞的强大探针， 细胞壁代谢 应用一个族的结果 葡聚糖和葡聚糖酶抗体将扩展到确定 组织暴露于葡萄糖醛酸阿拉伯木聚糖的后果 抗体的 阿魏酸的行为和双阿魏酸的作用 将评估与这些分子连接的酸。 3)一个重要 （2）的组成部分将是新酶机制的应用， 使用葡糖醛酸糖苷酶（以前称为feraxanase）， 葡糖醛酸阿拉伯聚糖，以确定结构（特别是 葡糖醛酸和阿魏酸组分）。 第二个重点 是基于这样的观察，即空间（定向）应用 抗体在抑制生长的能力方面很重要。 这一努力将有助于确定表皮的作用， 控制伸长。 结构和代谢方面也将 在分离的表皮细胞壁中进行检查。 实验 在这个建议中所描述的将有助于阐明分子 植物生长的机制。