Regulation of Carboxypeptidase in Mucor racemosus

总状毛霉羧肽酶的调控

• 批准号: 9106278
• 负责人: David Logan
• 金额: $ 21.5万
• 依托单位: Drexel University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-15 至 1995-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9106278&HistoricalAwards=false
• 关键词: Regulation; Carboxypeptidase; Mucor; racemosus

The fungus Mucor racemosus exists in both yeast-like and hyphal forms, and this dimorphism has associated with it several protease activities that appear morphology-specific. One of these, a serine carboxypeptidase termed CP3 is the focus of this research. The aims of the project include cloning and characterizing the structural gene that determines this protein, and to analyze the expression of that gene in cells undergoing rapid morphogenesis. The roles of proteolysis as regulatory mechanism(s) in cellular metabolism should be better understood from these studies.

真菌总状毛霉以酵母状和菌丝体的形式存在，这种二相性与其相关的几种蛋白酶活性似乎具有形态特异性。其中，一种名为CP3的丝氨酸羧肽酶是本研究的重点。该项目的目标包括克隆和鉴定决定这种蛋白质的结构基因，并分析该基因在快速形态发生过程中的细胞表达。从这些研究中可以更好地理解蛋白分解作为调节机制(S)在细胞代谢中的作用。