Intracellular Proteolysis and Biological Control

细胞内蛋白水解和生物控制

• 批准号: 8811788
• 负责人: David Logan
• 金额: $ 12万
• 依托单位: Drexel University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-10-15 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8811788&HistoricalAwards=false
• 关键词: Intracellular; Proteolysis; Biological; Control

The essential role of proteases in cellular control mechanisms warrants the identification of the proteases involved. We propose to investigate processes dependent upon protease action in the dimorphic fungus Mucor racemosus by combining biochemical and molecular biological techniques. During spore, swollen spore, and budding yeast-to-hyphal conversions, increases in aminopeptidase as well as carboxypeptidase levels were observed. Preliminary studies indicate that a 39 kilodalton protease (carboxypeptidase; CP2) is expressed in hyphal cells, but not in spore or yeast cells. We propose to isolate and study CP2 by using biochemical and protein chemical methods. The elucidation of the basic biochemistry of CP2 will provide a basis for developing hypotheses about its physiological functions. In addition, these studies should lay the foundation which will lead to our cloning and characterizing the CP2 gene. These investigations should increase the understanding of the relationship of protease to biological control processes in general and could provide information about the control of morphogenesis. These studies, using a fungal model system, are important to our understanding of the growth of this organism. In the long term, these studies have implications for our understanding of protein synthesis and metabolism, and nutrition in many organisms. Protein synthesis, function and transformation is vital in all organisms, yet we know little about the important biochemical events and their regulation. These studies are aimed at increasing our understanding of these important processes.

蛋白酶在细胞控制机制中的重要作用保证了所涉及的蛋白酶的鉴定。 我们建议通过结合生化和分子生物学技术来研究二态性真菌总状毛霉中依赖于蛋白酶作用的过程。 在孢子、膨胀孢子和出芽酵母向菌丝的转化过程中，观察到氨肽酶和羧肽酶水平增加。 初步研究表明，39 千道尔顿的蛋白酶（羧肽酶；CP2）在菌丝细胞中表达，但在孢子或酵母细胞中不表达。 我们建议通过生化和蛋白质化学方法分离和研究CP2。 CP2的基本生物化学的阐明将为提出关于其生理功能的假设提供基础。 此外，这些研究应该为我们克隆和表征CP2基因奠定基础。 这些研究应该增加对蛋白酶与生物控制过程之间关系的理解，并可以提供有关形态发生控制的信息。 这些使用真菌模型系统的研究对于我们了解这种生物体的生长非常重要。 从长远来看，这些研究对我们理解许多生物体中的蛋白质合成和代谢以及营养具有重要意义。 蛋白质的合成、功能和转化对于所有生物体都至关重要，但我们对重要的生化事件及其调控知之甚少。 这些研究旨在增加我们对这些重要过程的理解。