Intracellular Proteolysis and Biological Control

蛋白酶在细胞调控机制中的重要作用 保证了所涉及的蛋白酶的鉴定。 我们 建议研究依赖于蛋白酶作用的过程 通过结合生物化学方法， 和分子生物学技术。 在孢子期，肿胀 孢子和芽殖酵母到菌丝的转化， 观察氨肽酶以及羧肽酶水平。 初步研究表明一种39千道尔顿的蛋白酶 （羧肽酶; CP 2）在菌丝细胞中表达，但在菌丝细胞中不表达。 孢子或酵母细胞。 我们建议分离和研究CP 2， 使用生物化学和蛋白质化学方法。 阐明 CP 2的基本生物化学将为 对它的生理功能提出假说。 在 此外，这些研究应奠定基础， 我们克隆并鉴定了CP 2基因 这些 调查应增进对 蛋白酶与生物防治过程的关系 并可提供关于控制 形态发生 这些研究，使用真菌模型系统，对我们的研究很重要。 了解这种生物的生长。 从长远来看， 这些研究对我们理解蛋白质 合成和代谢，以及许多生物体的营养。 蛋白质的合成、功能和转化在所有 生物，但我们对重要的生物化学知之甚少。 事件及其监管。 这些研究旨在 加深我们对这些重要过程的理解。

The essential role of proteases in cellular control mechanisms warrants the identification of the proteases involved. We propose to investigate processes dependent upon protease action in the dimorphic fungus Mucor racemosus by combining biochemical and molecular biological techniques. During spore, swollen spore, and budding yeast-to-hyphal conversions, increases in aminopeptidase as well as carboxypeptidase levels were observed. Preliminary studies indicate that a 39 kilodalton protease (carboxypeptidase; CP2) is expressed in hyphal cells, but not in spore or yeast cells. We propose to isolate and study CP2 by using biochemical and protein chemical methods. The elucidation of the basic biochemistry of CP2 will provide a basis for developing hypotheses about its physiological functions. In addition, these studies should lay the foundation which will lead to our cloning and characterizing the CP2 gene. These investigations should increase the understanding of the relationship of protease to biological control processes in general and could provide information about the control of morphogenesis. These studies, using a fungal model system, are important to our understanding of the growth of this organism. In the long term, these studies have implications for our understanding of protein synthesis and metabolism, and nutrition in many organisms. Protein synthesis, function and transformation is vital in all organisms, yet we know little about the important biochemical events and their regulation. These studies are aimed at increasing our understanding of these important processes.