Primary Processes in Heliobacterial Reaction Center

幽门螺杆菌反应中心的初级流程

• 批准号: 9106685
• 负责人: Robert Blankenship
• 金额: $ 27.8万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9106685&HistoricalAwards=false
• 关键词: Primary; Processes; Heliobacterial; Reaction; Center

The heliobacteria, a newly discovered family of non-oxygen evolving photosynthetic bacteria, have a reaction center that is broadly similar to Photosystem I of oxygen evolving organisms. The goal of this project is to develop the heliobacteria as a bacterial model for Photosystem I in the same way that the purple photosynthetic bacterial have been very successfully used as a model for Photosystem II. Dr. Blankenship isolated the reaction center from Heliobacillus mobilis in an active form,, and is characterizing its chemical and physical properties using a variety of techniques. Preliminary femtosecond absorption difference spectra suggest that a 670 nm absorbing species is not the primary electron acceptor in the heliobacteria, in contrast to reports from other laboratories. Experiments are proposed to identify the chemical nature of the primary acceptor. Redox titrations indicate that a species that undergoes two electron reduction under some conditions is an early acceptor. This species is probably a menaquinone, analogous to the phylloquinone acceptor A1 in Photosystem I. Experiments are proposed to characterize this acceptor, as well as subsequent Fe-S protein acceptors. The properties of the membrane-bound cytochrome that acts as electron donor to the reaction center will also be investigated.

新发现的一个非氧细菌家族--日光菌 进化中的光合细菌，有一个反应中心， 与光系统I的氧释放过程大致相似 有机体 该项目的目标是开发 太阳细菌作为光系统I的细菌模型 就像紫色光合细菌 非常成功地用作光系统II的模型。 博士 布兰肯希普从太阳杆菌中分离出反应中心 mobilis在一个活跃的形式，并表征其化学 和物理性质。 初步的飞秒吸收差谱表明 670 nm的吸收物质不是初级电子 受体在太阳菌，相反，报告从 其他实验室。 实验提出了识别 主要受体的化学性质。 氧化还原滴定 表明一个经历两个电子还原的物种 在某些情况下是早期接受者。 本种 可能是甲萘醌，类似于叶绿醌受体 光系统I中的A1。提出实验来表征 该受体以及随后的Fe-S蛋白受体。 细胞色素的性质， 反应中心的电子供体也将是 研究了