Microbial Genome Sequencing: Genome Sequences for Four Phototrophic Prokaryotes

微生物基因组测序：四种光养原核生物的基因组序列

• 批准号: 0412824
• 负责人: Robert Blankenship
• 金额: $ 125.44万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-15 至 2007-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0412824&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Sequences; Four

Complete genome sequencing will be done for four photosynthetic bacteria. Genome sequences of these organisms will fill large gaps in the available genomic data for photosynthetic organisms and will help to understand the origin and early evolution of photosynthesis. In addition, the data that will be obtained will have agricultural applications and environmental importance for understanding global photosynthetic productivity. Photosynthesis has a deep evolutionary connection to nitrogen fixation and many photosynthetic bacteria are capable of nitrogen fixation. Our ability to understand these complex evolutionary relationships and metabolic processes is limited by a lack of data. This genome project will fill major gaps in the evolutionary picture of photosynthesis and its relation to other biological processes. The organisms that will be sequenced in this project are Heliobacterium modesticaldum, Roseobacter denitrificans, Rhodocista centenaria and Acaryochloris marina. The project will actively engage a large number of bioinformatics students in the annotation efforts. Bioinformatics graduate students will use the raw genome data as part of "real world" class exercises to identify metabolic pathways or families of transport proteins. The project team includes experts on each organism, including Robert Blankenship, Michael Madigan, Thomas Beatty, Carl Bauer, Mamoro Mimuro and Hideaki Miyashita and a highly experienced sequencing center, directed by Jeffrey Touchman. The team will also partner with the public science museum at the Arizona Science Center to develop public displays and teacher training materials aimed at communicating the excitement and benefits of microbial genomics to the general public and to school children, respectively.

将对四种光合细菌进行全基因组测序。这些生物的基因组序列将填补光合作用生物基因组数据的巨大空白，并将有助于了解光合作用的起源和早期进化。此外，将获得的数据将具有农业应用和环境重要性，以了解全球光合生产力。光合作用与固氮有着深刻的进化联系，许多光合细菌都具有固氮能力。我们理解这些复杂的进化关系和代谢过程的能力受到数据缺乏的限制。这个基因组计划将填补光合作用及其与其他生物过程的关系的进化图景中的主要空白。在这个项目中将要测序的生物是温和的太阳杆菌、反硝化玫瑰杆菌、百岁红杆菌和滨海绿心球藻。该项目将积极吸引大量生物信息学学生参与注释工作。生物信息学研究生将使用原始基因组数据作为“真实世界”课堂练习的一部分，以确定代谢途径或转运蛋白家族。项目团队包括每种生物的专家，包括Robert Blankenship， Michael Madigan, Thomas Beatty, Carl Bauer， Mamoro Mimuro和Hideaki Miyashita，以及由Jeffrey Touchman领导的经验丰富的测序中心。该团队还将与亚利桑那科学中心的公共科学博物馆合作，开发公共展示和教师培训材料，旨在分别向公众和学生传达微生物基因组学的兴奋和好处。