Simultaneous Physiology and 3-D Morphology of Neurons and Glial Cells

神经元和神经胶质细胞的同步生理学和 3D 形态学

• 批准号: 9108492
• 负责人: James Turner
• 金额: $ 26.9万
• 依托单位: Health Research Incorporated/New York State Department of Health
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-15 至 1995-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9108492&HistoricalAwards=false
• 关键词: Simultaneous; Physiology; Morphology; Neurons; Glial

This project will develop an integrated confocal light microscope and electrophysiology facility for observing living astroglia and neurons in cell culture, and neurons in tissue, providing the simultaneous recording of three-dimensional images and physiologic responses for different biochemical and physiologic stimuli. Two glial cell projects utilize a well characterized model system to study biochemical and morphologic changes due to receptor stimulation, mechanical stress, and ion uptake. The neuron studies utilize isolated ganglia from Aplysia and hippocampal slices. In Aplysia voltage-clamp measurements will be correlated with three-dimensional morphology for variations in media osmolarity, ionic strength, and composition. We have demonstrated volume changes under these conditions. Local circuits and cell-to-cell communication will be studied in hippocampal slices. All of the projects utilize confocal light microscopy, and when appropriate high-voltage electron microscopy.

本计画将发展一整合式共焦光学显微镜 和电生理学设施，用于观察活的星形胶质细胞， 细胞培养中的神经元和组织中的神经元， 同时记录三维图像， 不同生化和生理反应 刺激。 两个神经胶质细胞项目利用了一个良好表征的模型系统 研究受体引起的生物化学和形态学变化， 刺激、机械应力和离子吸收。 神经元 研究利用来自Astragsia和海马的分离神经节 切片 在失智症中，电压钳测量值将与 具有三维形态以适应介质的变化 渗透压、离子强度和组成。 我们有 在这些条件下显示体积变化。 当地 电路和细胞到细胞的通信将在 海马切片。 所有的项目都利用共焦光 显微镜，适当时高压电子 显微镜