Determination of the Mechanisms of Cell Adhesion to and Damage from Air-Liquid Interfaces

细胞粘附和气液界面损伤机制的确定

• 批准号: 9109151
• 负责人: Jeffrey Chalmers
• 金额: $ 13.73万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-15 至 1994-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9109151&HistoricalAwards=false
• 关键词: Determination; Mechanisms; Cell; Adhesion; Damage

The overall approach of the proposed work is to fundamentally understand the mechanisms of cell damage in contrast to the phenomenological/correlation type of approach which has been historically used to address this problem. The proposed approach requires the identification of the processes responsible for cell damage through initial visualization studies. Theoretical and correlational studies are then to be carried out in actual bioreactor operations to confirm that these mechanisms account for the observed cell damage. Once these mechanisms are confirmed, computer simulations of the hydrodynamic events, such as bubble rupture, will be used to determine the actual "strength" or "sensitivity" of cells to hydrodynamic forces.

所提出的工作的总体方法是从根本上理解细胞损伤的机制，而不是历史上用于解决这个问题的现象学/相关类型的方法。 所提出的方法需要通过最初的可视化研究来识别导致细胞损伤的过程。 然后在实际生物反应器操作中进行理论和相关研究，以确认这些机制解释了观察到的细胞损伤。 一旦这些机制得到证实，水动力事件（例如气泡破裂）的计算机模拟将用于确定细胞对水动力的实际“强度”或“敏感性”。