Light and Electron-Microscopic Studies of Auditory Hair-CellInnervation in the Leopard Frog

豹蛙听觉毛细胞神经支配的光和电子显微镜研究

• 批准号: 9110694
• 负责人: Dwayne Simmons
• 金额: $ 21.07万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9110694&HistoricalAwards=false
• 关键词: Light; Electron; Microscopic; Studies; Auditory

Frogs are vertebrates with two distinct auditory organs, rather than the single cochlea of mammals. A great deal is known about frog calling communication behavior, and about the physiology of frog auditory systems. However, unlike the case in mammals, the anatomical details and ultrastructure of how the sensory hair cells are innervated is poorly known. Such details are important in forming hypotheses about mechanisms for the observed frequency selectivity and physiology of frog hearing. This work will use light and electron microscopy to assess how the receptor cells are innervated by the auditory nerve in both these organs, and then to study the detailed architecture of the functional contancts, the synapses, of single nerve fibers in selected regions where function is known. The results will be important not only to comparative aspects of auditory function including physiology and behavior among species, but also to general principles of frequency coding mechanisms which are crucial to auditory neuroscience.

青蛙是脊椎动物，有两个不同的听觉器官，而不是哺乳动物的单一耳蜗。关于青蛙的叫声交流行为和青蛙听觉系统的生理机能，我们已经了解了很多。然而，与哺乳动物的情况不同，感觉毛细胞是如何被神经支配的解剖细节和超微结构尚不清楚。这些细节对于形成关于观察到的频率选择性和青蛙听觉生理机制的假设是重要的。这项工作将使用光镜和电子显微镜来评估这两个器官的受体细胞是如何被听神经支配的，然后研究功能接触的详细结构，突触，在选定的已知功能区域的单个神经纤维。这一结果不仅对物种间听觉功能的比较（包括生理和行为）具有重要意义，而且对听觉神经科学中至关重要的频率编码机制的一般原理也具有重要意义。