Oxytocinergic input to the sympathetic preganglionic neurons in the rat. A light and electron microscopic study using immunocytochemical technique.

大鼠交感神经节前神经元的催产素输入。

• 批准号: 06680730
• 负责人: HOSOYA Yasuhiko
• 金额: $ 1.41万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680730/
• 关键词: Symapthetic preganglionic neurons; Cholera toxin subunit B; Oxytocin; Immunohistochemistry; Rat

A combination of retrograde cell body labeling and immunohistochemistry was employed to elucidate how oxytocinergic fibers make contact with sympathetic preganglionic neurons (SPNs) in the rat spinal cord from T_1 to T_4. SPNs were labeled retrogradely using cholera toxin subunit B (CTb) or horseradish peroxidase conjugated CTb. Oxytocin immunoreactive (ir) fibers were found in the intermediate zone including the sympathetic preganglionic subnuclei. In the central autonomic nucleus and the intercalated nucleus, brown-stained oxytocin-ir varicosities or terminals were frequently observed to stud black-stained dendrites of SPNs. Electron microscopical observations showed that oxytocin-ir terminals form synapses with dendrites or soma of the sympathetic preganglionic neurons. The terminals contained numerous small clear round vesicles and a few large cored vesicles. These results clearly show that a large part of SPNs is innervated by oxytocin containing fibers. The origin of these fibers is discussed, and it is concluded that they are probably descending fibers from the paraventricular nucleus of the hypothalamus.

应用逆行胞体标记和免疫组织化学相结合的方法，研究了大鼠脊髓T_1 ~ T_4节段内催产素能纤维与交感节前神经元（SPNs）的接触。使用霍乱毒素亚单位B（CT B）或辣根过氧化物酶结合的CT B逆行标记SPN。催产素免疫反应阳性纤维位于交感神经节前亚核的中间区。在中央自主神经核和闰核内，黑染的SPN树突上常可见棕染的催产素阳性血管或终末。电镜观察显示催产素阳性终末与交感节前神经元的树突或索马体形成突触。末端含有许多小的透明圆形囊泡和一些大的核心囊泡。这些结果清楚地表明，大部分的SPN是由催产素含有纤维支配。本文讨论了这些纤维的来源，认为它们可能是下丘脑室旁核的下行纤维。

项目成果

細谷安彦: "交感神経節前細胞とオキシトシン陽性神経終末" 解剖学雑誌. 70. S181 (1995)

Yasuhiko Hosoya：“交感神经节前细胞和催产素阳性神经末梢”解剖学杂志 70. S181 (1995)。

Hosoya, Y.: "Thorocolumbar sympathetic preganglionic neurons in the dorsalcommissural nucleus of the male rat : an immunohistochemical study." 4th IBRO World Congress of Neuroscience. 437 (1995)

Hosoya，Y.：“雄性大鼠背连合核中的胸腰交感神经节前神经元：一项免疫组织化学研究。”

Hosoya, Y., Matukawa, M., Okado, N., Sugiura, Y., Kohno, K.: "Oxytocinergic innervation to the upper thoracic sympathetic preganglionic neurons in the rat. A light and electron microscopical study using a combined retrograde transport and immunocytochemic

Hosoya，Y.，Matukawa，M.，Okado，N.，Sugiura，Y.，Kohno，K.：“大鼠上胸交感神经节前神经元的催产素神经支配。使用组合逆行传输的光和电子显微镜研究

Hosoya, Y.: "Thorocolumbar sympathetic preganglionic neurons in the dorsal commissural nucleus of the male rat : an immunohistochemical study." 4th IBRO World Congress of Neuroscience. p437 (1995)

Hosoya，Y.：“雄性大鼠背连合核中的胸腰交感神经节前神经元：一项免疫组织化学研究。”

Hosoya.Y et al: "Thoracolumbar sympathetic preganglionic neurons in the dorsal commissurural nucleus of the male rat." Experimental Brain Research. 98. 21-30 (1994)

Hosoya.Y 等人：“雄性大鼠背连合核中的胸腰交感神经节前神经元。”