Cloning of the TuMV Genome and Analysis of Its Movement Protein

TuMV基因组克隆及其运动蛋白分析

• 批准号: 9112587
• 负责人: Jerry Bryant
• 金额: $ 1.2万
• 依托单位: University of Missouri-Saint Louis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-15 至 1993-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9112587&HistoricalAwards=false
• 关键词: Cloning; TuMV; Genome; Analysis; Its

The proposed planning activity will enable the applicant to prepare a cDNA library of the Turnip mosaic virus and develop viral mutants and probes as well as other reagents required for a longer term research plan directed toward establishing the gene sequence, the protein structure and the function of the turnip mosaic virus movement protein. Turnip mosaic virus is a member of an agriculturally important and widespread group of plant viruses. These viruses occur worldwide and infect all vegetable and ornamental plants of the mustard and cress family. These viruses are transmitted by many species of aphid. When viruses are inoculated onto host plants, either by a feeding insect or by an abrasion, the initial infection is limited to a single or few cells of the plant. In order for the virus to successfully infect the entire plant, movement from cell to cell is necessary. This movement involves the plasmodesmata, which are membrane-lined pores extending through the cell walls and connecting adjacent plant cells. A virus-encoded factor termed the "movement protein" is also required. The movement protein of another plant virus has been shown to increase the effective size of the channels between cells, and this is thought to facilitate movement of virus from cell to cell. The movement protein of turnip mosaic virus probably has a similar function. The wide- ranging turnip mosaic virus and its movement protein are excellent candidates for genetic manipulation for crop protection, but the molecular biology of this system also has the potential for providing new information on the biology of plant-virus interactions, the structure and function of the plasmodesmata and the molecular biology of plant virus replication. The goal of the proposed planning activity is to lay the experimental groundwork for determining the structure of the gene encoding the turnip mosaic virus movement protein and ultimately studying its function.

拟议的规划活动将使申请人能够准备萝卜花叶病毒的cDNA文库，并开发病毒突变体和探针以及长期研究计划所需的其他试剂，该长期研究计划旨在建立萝卜花叶病毒运动蛋白的基因序列、蛋白质结构和功能。 芜菁花叶病毒是农业上重要且广泛传播的植物病毒组的一员。 这些病毒在世界范围内发生并感染芥菜和水芹科的所有蔬菜和观赏植物。 这些病毒由多种蚜虫传播。 当病毒通过进食昆虫或擦伤而接种到宿主植物上时，最初的感染仅限于植物的单个或几个细胞。 为了使病毒成功感染整个植物，需要从一个细胞移动到另一个细胞。 这种运动涉及胞间连丝，胞间连丝是延伸穿过细胞壁并连接相邻植物细胞的膜内孔。 还需要一种被称为“运动蛋白”的病毒编码因子。 另一种植物病毒的运动蛋白已被证明可以增加细胞之间通道的有效尺寸，这被认为有助于病毒在细胞之间的运动。 萝卜花叶病毒的运动蛋白可能具有类似的功能。 范围广泛的萝卜花叶病毒及其运动蛋白是作物保护遗传操作的绝佳候选者，但该系统的分子生物学也有可能为植物-病毒相互作用的生物学、胞间连丝的结构和功能以及植物病毒复制的分子生物学提供新信息。 拟议规划活动的目标是为确定编码萝卜花叶病毒运动蛋白的基因的结构并最终研究其功能奠定实验基础。